To compare the application value of immunofluorescence and sialidase in the diagnosis of vaginal Gardnerella vaginalis.

Total of 456 vaginal secretions samples from married women were tested by immunofluorescence and sialidase. Amsel was taken as the "gold standard" , the diagnostic performance of the two method were compared.

There were 175 cases among the 456 tested women were detected GV positive, with the positive rate of 38.38% (175/456). The detection rate of 18-29 years old was 42.79% (95/222), which was significantly higher than that of 40-49 years old group (χ² = 5.807, P = 0.016). The detection rate of patients with bacterial vaginosis was significantly higher than those of infertility, adverse pregnancy and other people (χ² = 13.860, P = 0.000; χ² = 21.131, P = 0.000; χ² = 13.860, P = 21.492). There were 181 cases detected GV positive by immunofluorescence detection with 13 cases of false positive, while 177 cases were detected GV positive by sialidase detetion with 21 cases of false positive. The sensitivity, specific, accuracy, positive predictive value and negative predictive value of the immunofluorescence detection were 96.00%, 95.37%, 95.61%, 95.37% and 97.45%, while those of the sialidase detection were 89.14%, 92.53%, 91.23%, 88.14% and 93.19%. The sensitivity, accuracy, negative predictive value of immunofluorescence were significantly higher than those of sialidase (χ² = 5.583, P = 0.014; χ² = 7.136, P = 0.008; χ² = 5.631, P = 0.0180).

The infection rate of VG varies in patients with different ages and has high sensitivity, accuracy, negative predictive value, and could be used for vaginal Gardner fungus infection in patients with bacterial vaginal disease screening.