Progression of laboratory diagnosis of measles

doi:10.3877/cma.j.issn.1674-1358.2016.03.002

Abstract

Abstract:

Measles is a serious infectious disease, even to now it could be widespread occasionally in some places. The diagnosis of measles could not only depend on clinical symptoms, also laboratory examinations should be necessary. MV-specific IgM antibody could be detected reliably by ELISA, but often with false negative in the earlier course of disease. The one-step real-time RT-PCR assay was a simple and sensitive method for MV diagnosis within 2 hours. It needs further study that use a specific antibody to find out MV in brushing off celles by indirect immunofluorescent assay. Measles virus isolation and plaque neutralisation assays weren’t suitable for making routine diagnosis.

Key words: Measles virus (MV), Enzyme linked immunosorbent assay (ELISA), Real-time RT-PCR assay, Laboratory Diagnosis, Indirect immunofloresence assay (IFA)

Jun Xu, Xiao Zhao, Cunjun Hou. Progression of laboratory diagnosis of measles[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(03): 261-264.

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