Expression of T cell subsets in children with hand, foot and mouth disease caused by enterovirus 71 infection

doi:10.3877/cma.j.issn.1674-1358.2018.04.016

Abstract

Abstract:

Objective

To investigate the expression of T cell subsets in children with hand, foot and mouth disease (HFMD) of EV71 infection.

Methods

From October 2015 to October 2017, 302 children with HFMD were treated in Huizhou City People’s Hospital, including 110 cases with EV71 infection. According to whether or not the central nervous system was involved in children with EV71 infection, cases were divided into mild group (69 cases) and severe group (41 cases), while 110 healthy children were selected as the control group. T lymphocyte subsets CD3⁺, CD3⁺CD8^-, CD3⁺CD8⁺, CD3⁺CD8^-/CD3⁺CD8⁺ , Th1, Th2, Th1/Th2, Tc1, Tc2, Tc1/Tc2, Th17, Tc17 and regulatory T cells (Treg) and Th17/Treg ratio were detected by flow cytometry, respectively. The change of serum TGF-beta 1 (TGF-β1), interleukin-4 (IL-4), interleukin-17A (IL-17A) and interferon gamma (IFN-γ) were detected by ELISA.

Results

The levels of serum Th1, Th1/Th2, Tc1 and Tc1/Tc2 of cases in mild group were (9.59 ± 2.15)%, (8.67 ± 2.19)%, (28.82 ± 5.49)% and (56.39 ± 10.48)%; while those in severe group were (13.58 ± 2.74)%, (9.45 ± 2.29)%, (40.46 ± 6.37)% and (54.87 ± 9.61)%, respectively, which were all significantly higher than those of the control group, with statistically significant differences (F= 12.159, 11.470, 13.925, 10.542,P= 0.016, 0.013, 0.008, 0.035). The levels of serum IFN-γ and IL-17A levels of cases in mild group were (11.32 ± 2.76) pg/ml, (6.38 ± 2.10) pg/ml, while in severe group were (11.38 ± 2.65) pg/ml, (12.59 ± 3.58) pg/ml, which were all significantly higher than those of the control group, with significant differences (F= 12.590, 11.664;P= 0.024, 0.019).

Conclusion

The proportion of Th17/Treg and Th1/Th2 in children with HFMD after EV71 infection is unbalanced.

Key words: Hand foot and mouth disease, Enterovirus 71, T cell subgroup

Li Zou, Wenjing Mei. Expression of T cell subsets in children with hand, foot and mouth disease caused by enterovirus 71 infection[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2018, 12(04): 397-401.

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Figures/Tables 6

References 25

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组别	例数	男/女（例）	平均年龄（岁）	最高体温（℃）	发病持续时间（d）	中性粒细胞（%）	WBC（× 10⁹/L）	CRP（mg/L）
轻症组	69	47/22	2.08 ± 0.93	38.62 ± 0.42	2.70 ± 1.24	59.06 ± 13.72	10.28 ± 2.16	5.97 ± 2.49
重症组	41	26/15	1.97 ± 0.86	38.78 ± 0.49	4.31 ± 1.46	63.58 ± 16.43	12.26 ± 2.58	8.04 ± 3.45
对照组	110	63/47	2.05 ± 0.84	—	—	62.07 ± 8.51	6.21 ± 0.37	—
统计量	?	χ² = 1.027	F= 2.119	F= 0.983	F= 10.481	F= 1.697	F= 11.452	F= 2.067
P值	?	0.183	0.229	0.604	0.032	0.315	0.025	0.174

组别	例数	男/女（例）	平均年龄（岁）	最高体温（℃）	发病持续时间（d）	中性粒细胞（%）	WBC（× 10⁹/L）	CRP（mg/L）
轻症组	69	47/22	2.08 ± 0.93	38.62 ± 0.42	2.70 ± 1.24	59.06 ± 13.72	10.28 ± 2.16	5.97 ± 2.49
重症组	41	26/15	1.97 ± 0.86	38.78 ± 0.49	4.31 ± 1.46	63.58 ± 16.43	12.26 ± 2.58	8.04 ± 3.45
对照组	110	63/47	2.05 ± 0.84	—	—	62.07 ± 8.51	6.21 ± 0.37	—
统计量	?	χ² = 1.027	F= 2.119	F= 0.983	F= 10.481	F= 1.697	F= 11.452	F= 2.067
P值	?	0.183	0.229	0.604	0.032	0.315	0.025	0.174

组别	例数	CD3⁺	CD3⁺CD8^－	CD3⁺CD8⁺	CD3⁺CD8^－/CD3⁺CD8⁺
轻症组	69	63.80 ± 10.75	34.52 ± 9.03	24.69 ± 6.28	1.49 ± 0.21
重症组	41	65.08 ± 9.76	33.71 ± 7.95	26.27 ± 6.89	1.36 ± 0.18
对照组	110	66.62 ± 9.57	35.98 ± 9.37	24.95 ± 7.04	1.45 ± 0.19
F值	?	0.895	1.637	2.115	1.492
P值	?	0.193	0.274	0.182	0.317

组别	例数	CD3⁺	CD3⁺CD8^－	CD3⁺CD8⁺	CD3⁺CD8^－/CD3⁺CD8⁺
轻症组	69	63.80 ± 10.75	34.52 ± 9.03	24.69 ± 6.28	1.49 ± 0.21
重症组	41	65.08 ± 9.76	33.71 ± 7.95	26.27 ± 6.89	1.36 ± 0.18
对照组	110	66.62 ± 9.57	35.98 ± 9.37	24.95 ± 7.04	1.45 ± 0.19
F值	?	0.895	1.637	2.115	1.492
P值	?	0.193	0.274	0.182	0.317

组别	例数	Th17	Tc17	Treg	Th17/Treg
轻症组	69	1.33 ± 0.24	0.62 ± 0.16	0.81 ± 0.17	1.39 ± 0.36
重症组	41	2.36 ± 0.61	1.02 ± 0.24	0.98 ± 0.23	2.90 ± 0.67
对照组	110	0.59 ± 0.12	0.29 ± 0.05	0.79 ± 0.21	0.87 ± 0.24
F值	?	13.255	14.691	2.108	12.450
P值	?	0.006	0.009	0.281	0.016

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