To investigate the case of a tertiary level hospital staff infectious blood borne occupational exposure, the differences related knowledge and protective behaviors of the different hospital staff blood borne occupational exposure, and to provide a reference for the overall control mode of exploring the hospital occupational protection and safety.

Exposure of the hospital management were investigated by taking a questionnaire occupational exposure protection awareness. At this stage, 210 medical staff in the hospital was collected by random sampling for the questionnaire survey, while 196 valid questionnaires were collected. The questionnaire were composed of the medical staff’s population sociology data, the occupational exposure cognitive status of the blood source, occupational exposure management behavior of the hospital, occupational exposure status and occupational protection behavior.

The average score of health care workers’ exposure to blood source occupational exposure was 8.11 points. Better overall cognitive, different departments, different titles, different age groups and gender on the medical staff occupational exposure management cognitive, with significant differences (all P < 0.05), but different education levels and working-age cognitive between occupational exposure management had no significant differences. However, there was no significant difference between different academic degrees and occupational exposure management. The proportion of medical staff in the hospital who were not vaccinated against hepatitis B was still 10.20%, and 14.80% were not concerned about or had never been considered. There were 89.00% of the medical personnel compares a standard exposure after emergency treatment, 97 medical staff occupational exposure were 80.41% (78 cases) of medical staff report in a timely manner, and insist on monitoring and follow-up after exposure.

The professional protection cognition of the hospital and the emergency treatment and report after occupational exposure have been better, but still need to continue to improve the hospital infection management ability and health care personnel’s awareness of prevention.