To investigate the drug resistance to antimicrobial of clinical isolates in Rushan People’s Hospital in 2015, and to provide basis for rational drug use and hospital infection control.

Disk diffusion method (K-B method) was used to detect susceptibility according to CLSI 2014. Data was analyzed by WHIONET 5.4 software.

Total of 2 596 non-repeating isolates were collected in clinical specimen in 2015. There were 661 (25.5%) isolates were Gram-positive cocci, 1 935 (74.5%) were Gram-negative bacilli. Staphylococcus aureus and Escherichia coli were the most isolates in the Gram-positive and Gram-negative bacteria, respectively. Methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis were 23.0% and 73.6% in Staphylococcus, respectively. Vancomycin-resistant Staphylococcus and Enterococcus were not detected. Most Gram-negative bacteria were resistant to ampicillin and ciprofloxac, while sensitive to carbapenems and compound preparation containing inhibitors. Escherichia coli and Klebsiella pneumoniae producing Extended-Spectrum β-lactamase (ESBL) were 37.4% and 25.4%, respectively. ESBL producing strains could often lead to multi-drug resistance.

The most pathogens isolated in our hospital were E. coli. The resistance of E. coli. producing ESBL and methicillin-resistant Staphylococcus aureus was significant, which should be paid attention to by clinicians and hospital infection department.