To investigate the clinical distribution of isolated Escherichia coli and its resistance to common antimicrobial agents and the production of carbapenemases.

Various strains of 298 patients from Wuhan University People’s Hospital from June 2014 to February 2016 were collected. The strains were identified by BD Phoenix^TM 100 bacterial identification and drug susceptibility system. On The strains of alkene antimicrobial with sensitivity decreasing to carbon penicillium were confirmed by Carba NP, genotypes were determined by PCR. The MLST technique of Enterobacter cloacae was applied for molecular typing.

Total of 298 strains of Enterobacter cloacae were detected, among which the samples were mainly from sputum (29.87%), followed by urine (25.50%). In the departments distribution, neurosurgery (17.79%) was the most, followed by hepatobiliary surgery (12.75%). The resistance rate of Enterobacter cloacae to cefazolin was 98.1%, followed by ampicillin (89.7%). Among the strains resistant to carbapenem antibiotics, 9 strains were detected by Carba NP test, 8 of which were positive, one strain was negative. Four strains of NDM-1 gene were amplified by PCR. One strain carrying the VIM-19 gene and one carrying the VIM-19 gene alone were detected by sequencing. Four strains of NDM-1 carbapenem were classified by MLST, and 3 different ST types were obtained, which were ST508, ST509 and ST145, respectively.

Antibiotic resistance of Enterobacter cloacae was grim, infection control departments should strengthen the monitoring of carbapenem to reduce the emergence and spread of drug-resistant bacteria.