The molecular characteristics of bacillary dysentery epidemic and the antibiotic resistance mechanisms of plasmid mediated quinolones in Shenzhen

doi:10.3877/cma.j.issn.1674-1358.2016.03.007

Abstract

Abstract:

Objective

To analyze the molecular characteristics of bacillary dysentery epidemic and the antibiotic resistance mechanisms of plasmid mediated quinolones in Shenzhen.

Methods

Clinical specimens were collected in 18 hospitals in Shenzhen form January 2010 to Febuary 2014 and isolation cultivation and serotype identification were applied. The plasmid mediated main type of quinolones genes were detected by PCR. Minimal inhibitory concentration (MIC) was tested by Agar dilution method. Transcojugants genotype and drug resistance were tested by joint transfer experiment.

Results

Serological distribution: during all 126 strains of Shigella, there were 108 (87.51%) strains of Shigella flexneri and 16 (12.70%) strains of Shigella sonnei. The superiority serotype of shigella flexneri was serum Ⅳ-C, with 42 strains counted for 38.89%. Commonly used antimicrobial susceptibility situation analysis: the sensitivity of Shigella flexneri to NAl, FEP and GM were significantly lower than that of Shigella sonnei, while the sensitivity of Shigella flexneri to LEV, GIP, NOR, CAZ and AMC were significantly higher than that of Shigella sonnei (P all < 0.05). Amplification results and sequence analysis: 3 (2.38%) cases with qnr genes, 4 cases with aac6’ genes, and 1 case with qepA genes were checked out in 126 strains Shigella. Minimum inhibitory concentration: compared with receptor bacteria, the MIC of transconjugants on NAL, GIP, LEV, NOR, GM were improved by 2-32 times.

Conclusions

The main types of Shigella infection in Shenzhen are Shigella flexneri and Shigella sonnei. The superiority serotype of shigella flexneri is Ⅳ-C. Target gene mutation is the main cause of quinolones resistance. The quinolones resistance of different types of shigella varies significantly.

Key words: Bacillary dysentery, Shigella, Plasmid mediated, Quinolones, Drug-resistance

Changzheng Cai, Shaowei Shu, Aiping Chen, Guoqing Huang, Meirong Zhou. The molecular characteristics of bacillary dysentery epidemic and the antibiotic resistance mechanisms of plasmid mediated quinolones in Shenzhen[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(03): 285-289.

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References 25

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引物名称	引物序列	退火温度（℃）	延伸时间（s）	产物长度（bp）
aac（6’）	F：TTGCGATGCTCTATGAGTGG	55	60	580
aac（6’）	R：CTCGAATGGCCTGGCGTGTTT	55	60	580
qepA	F：CGTGTTGCTGGAGTTCTTC	55	60	405
qepA	R：CTGCAGGTACTGCGTCATG	55	60	405
Qnr B4	F：ATCACCACCCGCACCT	50	60	242
Qnr B4	R：TCTAAATCGCCCAGTTCC	50	60	242
Qnr A	F：TCGGCAAAGGTCAGGTCACAGC	48	60	480
Qnr A	R：GCAAGAGGATTTCTCACGCCAGGAT	48	60	480

引物名称	引物序列	退火温度（℃）	延伸时间（s）	产物长度（bp）
aac（6’）	F：TTGCGATGCTCTATGAGTGG	55	60	580
aac（6’）	R：CTCGAATGGCCTGGCGTGTTT	55	60	580
qepA	F：CGTGTTGCTGGAGTTCTTC	55	60	405
qepA	R：CTGCAGGTACTGCGTCATG	55	60	405
Qnr B4	F：ATCACCACCCGCACCT	50	60	242
Qnr B4	R：TCTAAATCGCCCAGTTCC	50	60	242
Qnr A	F：TCGGCAAAGGTCAGGTCACAGC	48	60	480
Qnr A	R：GCAAGAGGATTTCTCACGCCAGGAT	48	60	480

抗菌药物	福氏志贺菌（n = 108）		宋氏志贺菌（n = 16）		χ²值	P值
抗菌药物	敏感株数	敏感率（%）	敏感株数	敏感率（%）	χ²值	P值
萘啶酸	13	12.04	7	43.75	10.361	< 0.01
左氧氟沙星	108	100.00	11	68.75	35.168	< 0.01
环丙沙星	97	89.81	7	43.75	21.859	< 0.01
诺氟沙星	108	100.00	7	43.75	65.504	< 0.01
氧氟沙星	107	99.07	7	43.75	57.528	< 0.01
头孢他啶	104	96.30	6	37.50	42.409	< 0.01
头孢吡肟	13	12.04	8	50.00	11.706	< 0.01
亚胺培南	108	100.00	16	100.00	—	—
阿莫西林	108	100.00	14	87.50	6.975	< 0.05
庆大霉素	6	5.56	12	75.00	48.706	< 0.01

抗菌药物	福氏志贺菌（n = 108）		宋氏志贺菌（n = 16）		χ²值	P值
抗菌药物	敏感株数	敏感率（%）	敏感株数	敏感率（%）	χ²值	P值
萘啶酸	13	12.04	7	43.75	10.361	< 0.01
左氧氟沙星	108	100.00	11	68.75	35.168	< 0.01
环丙沙星	97	89.81	7	43.75	21.859	< 0.01
诺氟沙星	108	100.00	7	43.75	65.504	< 0.01
氧氟沙星	107	99.07	7	43.75	57.528	< 0.01
头孢他啶	104	96.30	6	37.50	42.409	< 0.01
头孢吡肟	13	12.04	8	50.00	11.706	< 0.01
亚胺培南	108	100.00	16	100.00	—	—
阿莫西林	108	100.00	14	87.50	6.975	< 0.05
庆大霉素	6	5.56	12	75.00	48.706	< 0.01

抗菌药物	临床株			J53Az	接合子
抗菌药物	S12 aac（6’）	S21 qepA	S69 qnr	J53Az	T12 aac（6’）	T21 qepA	T69 qnr
萘啶酸	> 256	> 256	> 256	2	4	4	8
环丙沙星	2	> 32	16	< 0.0625	0.50	0.25	0.50
左氧氟沙星	1	16	16	< 0.125	2	1	0.5
诺氟沙星	8	> 128	32	< 0.25	0.25	0.50	1
庆大酶素	> 128	> 128	128	1	16	16	8
亚胺培南	< 0.25	< 0.25	< 0.25	< 0.25	< 0.25	< 0.25	< 0.25

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