Identification and phylogenetic analysis of Rothia aeria isolated from blood of a patient with fever

doi:10.3877/cma.j.issn.1674-1358.2016.02.026

Abstract

Abstract:

Objective

To investigate the clinical and laboratory features of bloodstream infection caused by Rothia aeria, and to supply better diagnosis and therapy.

Methods

The clinical diagnosis, treatments, outcomes and laboratory data, including blood culture, identification, antimicrobial susceptibility testing results were reviewed and analyzed, respectively. The bacterial identification was taken by 16S rRNA sequencing and MALDI Biotyper mass spectrometry.

Results

Rothia aeria bloodstream infection was the first report in China (Genbank login number for KF691779). MALDI Biotyper mass spectrometry and 16S rRNA gene sequencing could identify Rothia aeria accurately. The results of antimicrobial susceptibility testing by E-test method showed that levofloxacin, penicillin, ampicillin, erythromycin, gentamicin, imipenem, piperacillin and ceftazidime were all sensitive. Ampicillin/sulbactam were used with high-dose and long-term treatment, the patient was discharged.

Conclusions

The clinical infections caused by Rothia aeria are rare. 16S rRNA and MALDI Biotyper mass spectrometry could accurately identify Rothia aeria. Blood culture is of important value for pathogen detection.

Key words: Blood culture, Rothia aeria, 16S rRNA, Phylogenetic analysis, MALDI biotyper mass spectrometry

Jingrong Cao, Peichang Wang, Dingxia Shen, Rong Min, Lili Zhang, Yuying Wang, Yan Wang. Identification and phylogenetic analysis of Rothia aeria isolated from blood of a patient with fever[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(02): 254-256.

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References 13

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