To investigate the distribution and antibacterial resistance of nosocomial infection pathogens from a new private general hospital, and to provide reference for clinical rational use of antibacterial agents.

The data of pathogens formation, distribution and antibacterial resistance of a new general private hospital from January 1st 2008 to December 30th 2011, which included 3 728 strains were analyzed by χ² test, making a comparison with CHINET 2010 surveillance of bacterial resisitance in China and the new grade-three hospital.

Among all the strains, Gram-negative bacteria and Gram-positive bacteria accounted for 79.8% and 20.2%, respectively, of which Escherichia coli, Pseudomonas aeruginosa, Klebsiella, Acinetobacter, Staphylococcus aureus and coagulase-negative Staphyococcus were common in the Gram-negative bacteria, and Klebsiella were highly sensitive to cefoperazone-sulbactam, imipenem and meropenem, with the resistant rate of 0%, significantly lower than those of CHINET 2010 in China, with significant differences (P all < 0.01). The resistance rates of Escherichia coli to the three drugs were 1.52%, 1.2% and 1.39%, lower than those of the CHINET results, and the resistance rates to cefoperazone-sulbactam were with significant differences (P all < 0.01), and the resistant rates to imipenem and meropenem were not significantly different (P = 0.315 and 0.988). The resistance rates of Pseudomonas aeruginosa to the above three drugs were 16.96%, 22.84% and 29.3%, while the resistance rate to cefoperazone-sulbactam was not significantly different (P = 0.536), the resistance rate to imipenem and meropenem were significantly different (P < 0.01 and < 0.05). The resistance rates of Acinetobacter to the three drugs were 4.5%, 47.5% and 39.9%, significantly lower than those of CHINET results (P all < 0.01). Gram-positive cocci to vancomycin, teicoplanin and linezolid were highly sensitive, with the resistance rates of 0%, which was same to the CHINET results. Compared with the drug resistance rates of the West District of Beijing Chaoyang Hospital, the resistance rates of Pseudomons asaeruginosa to gentamicin, piperacillin-tazobactam and meropenem were 67.45%, 36.69% and 29.3%, which were significantly higher than those of the West District of Beijing Chaoyang Hospital (P all < 0.01). The resistance rates of Escherichia coli were similar in the two hospital, with no significant differences (P > 0.05). Compared with the resistance rate of Gram-positive cocci in the West District of Beijing Chaoyang Hospital, and Staphyococcus resistance rate was significantly lower (P all < 0.01).

Formation, distribution and antibacterial resistance of the new general private hospital’s nosocomial infection pathogens were consistent with the domestic related research results.