了解天门地区手足口病（HFMD）疑似患儿CoxA16和EV71的感染情况，为其有效治疗和干预提供依据。

收集住院及门诊HFMD疑似患儿血标本，应用ELISA法测定血清中CoxA16-IgM和EV71-IgM含量。

共收集3 009例HFMD疑似患儿，总阳性率13.2%（396/3 009），CoxA16-IgM阳性率为10.77%（149/1 384），EV71-IgM阳性率为15.2%（247/1 625），CoxA16 + EV71混合感染阳性率为10.57%（146/1 381）。单纯感染与混合感染男性患儿均显著多于女性患儿，差异均具有统计学意义（χ²_EV71 = 4.60、P = 0.017，χ²_CoxA16 = 4.64、P = 0.025，χ²_{EV71 + CoxA16} = 4.36、P = 0.030）；1岁患儿组阳性率最高（较其他各年龄组，P均= 0.000）。

天门地区儿童HFMD感染率较高，病原以EV71为主，以CoxA16 + EV71混合感染较严重，疾控部门应引起足够重视，防止HFMD的暴发流行。

To investigate the infection of Coxsaekie virus A16 (CoxA16) and entervirus 71 (EV71) about hand, foot and mouth disease (HFMD) suspected child in Tianmen, and to provide reference for better treatment and intervention.

The blood specimens of children suspected with hand, foot and mouth disease were collected, and then CoxA16-IgM and EV71-IgM antibody in serum were detected by ELISA.

Total of 3 009 cases of HFMD suspected children were collected, the total of positive rate was 13.2% (396/3 009), the positive rate of CoxA16-IgM was 10.77% (149/1 384), the positive rate of EV71-IgM was 15.2% (247/1 625), the positive rate of CoxA16-IgM + EV71-IgM co-infection was 10.77% (146/1 381). The boy’s amount was larger than the girl’s in both simple infection and the mixed infection, both with significant differences (χ²_EV71 = 4.60, P = 0.017; χ²_CoxA16 = 4.64, P = 0.025; χ²_{EV71 + CoxA16} = 4.36, P = 0.030). The highest positive rate was patients in one years old group (compared with other groups, P all = 0.000).

The infection rate of HFMD in Tianmen was very high, and the major pathogen was EV71. It was very serious that mixed infection was CoxA16 + EV71, thus, department for disease prevention and control should pay more attention to HFMD and prevent HFMD outbreaking.