比较免疫荧光法与唾液酶法在阴道检测加德纳菌感染诊断中的应用价值。

采集来本院门诊就诊或体验的已婚妇女456例阴道分泌物标本，分别采用免疫荧光法与唾液酸酶法检测，以Amsel检测法为"金标准"，比较两种检测方法的诊断效能。

456例受检妇女中，检出阴道加德纳菌阳性175例，检出率为38.38%（175/456）；18～29岁年龄段检出率为42.79%（95/222），显著高于40～49岁年龄段（χ² = 5.807，P = 0.016）；细菌性阴道病患者加德纳菌阳性检出率显著高于不孕不育、不良怀孕及其他人群，差异具有统计学意义（χ² = 13.860、P = 0.000，χ² = 21.131、P = 0.000，χ² = 13.860、P = 21.492）；免疫荧光法检出阴道加德纳菌阳性181例（其中假阳性13例），唾液酸酶法检测阴道加德纳菌阳性177例（其中假阳性21例）；免疫荧光法检测灵敏度、特异度、准确度、阳性预测值和阴性预测值分别为96.00%、95.37%、95.61%、92.82%和97.45%；唾液酸酶法检测灵敏度、特异度、准确度、阳性预测值和阴性预测值分别为89.14%、92.53%、91.23%、88.14%和93.19；免疫荧光法灵敏度、准确度和阴性预测值显著高于唾液酸酶法，差异均具有统计学意义（χ² = 5.583、P = 0.014，χ² = 7.136、P = 0.008，χ² = 5.631、P = 0.018）。

不同年龄段、不同受检对象阴道加德纳菌感染率各不相同，免疫荧光法具有较高灵敏度、准确度和阴性预测值，可用于细菌性阴道病阴道加德纳菌感染者的筛查。

To compare the application value of immunofluorescence and sialidase in the diagnosis of vaginal Gardnerella vaginalis.

Total of 456 vaginal secretions samples from married women were tested by immunofluorescence and sialidase. Amsel was taken as the "gold standard" , the diagnostic performance of the two method were compared.

There were 175 cases among the 456 tested women were detected GV positive, with the positive rate of 38.38% (175/456). The detection rate of 18-29 years old was 42.79% (95/222), which was significantly higher than that of 40-49 years old group (χ² = 5.807, P = 0.016). The detection rate of patients with bacterial vaginosis was significantly higher than those of infertility, adverse pregnancy and other people (χ² = 13.860, P = 0.000; χ² = 21.131, P = 0.000; χ² = 13.860, P = 21.492). There were 181 cases detected GV positive by immunofluorescence detection with 13 cases of false positive, while 177 cases were detected GV positive by sialidase detetion with 21 cases of false positive. The sensitivity, specific, accuracy, positive predictive value and negative predictive value of the immunofluorescence detection were 96.00%, 95.37%, 95.61%, 95.37% and 97.45%, while those of the sialidase detection were 89.14%, 92.53%, 91.23%, 88.14% and 93.19%. The sensitivity, accuracy, negative predictive value of immunofluorescence were significantly higher than those of sialidase (χ² = 5.583, P = 0.014; χ² = 7.136, P = 0.008; χ² = 5.631, P = 0.0180).

The infection rate of VG varies in patients with different ages and has high sensitivity, accuracy, negative predictive value, and could be used for vaginal Gardner fungus infection in patients with bacterial vaginal disease screening.