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中华实验和临床感染病杂志(电子版) ›› 2016, Vol. 10 ›› Issue (03) : 261 -264. doi: 10.3877/cma.j.issn.1674-1358.2016.03.002

综述

麻疹的实验室诊断研究进展
徐军1, 赵晓1, 侯存军2,()   
  1. 1. 264100 烟台市,烟台市传染病医院体检中心
    2. 264100 烟台市,烟台市传染病医院皮肤性病科
  • 收稿日期:2015-06-27 出版日期:2016-06-15
  • 通信作者: 侯存军
  • 基金资助:
    烟台市科技发展计划项目(No. 2011249)

Progression of laboratory diagnosis of measles

Jun Xu1, Xiao Zhao1, Cunjun Hou2,()   

  1. 1. Department of Physical Examination Center, Yantai City Hospital of Infectious Diseases, Yantai 264100, China
    2. Department of Dermatology and Venerology, Yantai City Hospital of Infectious Diseases, Yantai 264100, China
  • Received:2015-06-27 Published:2016-06-15
  • Corresponding author: Cunjun Hou
引用本文:

徐军, 赵晓, 侯存军. 麻疹的实验室诊断研究进展[J]. 中华实验和临床感染病杂志(电子版), 2016, 10(03): 261-264.

Jun Xu, Xiao Zhao, Cunjun Hou. Progression of laboratory diagnosis of measles[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(03): 261-264.

麻疹是一种致病力很强的传染病,当前仍不时有疫情暴发。其临床诊断准确率不高,需实验室检查辅助确诊。麻疹病毒IgM抗体检测易操作,结果可靠,但发病早期易出现假阴性。一步法实时荧光定量RT-PCR能在发病早期检出病毒RNA。操作简单、快速、结果敏感。使用单克隆抗体间接免疫荧光法检测脱落细胞内麻疹病毒抗原的可靠性有待进一步研究。病毒分离和蚀斑中和实验不宜作为常规诊断使用。

Measles is a serious infectious disease, even to now it could be widespread occasionally in some places. The diagnosis of measles could not only depend on clinical symptoms, also laboratory examinations should be necessary. MV-specific IgM antibody could be detected reliably by ELISA, but often with false negative in the earlier course of disease. The one-step real-time RT-PCR assay was a simple and sensitive method for MV diagnosis within 2 hours. It needs further study that use a specific antibody to find out MV in brushing off celles by indirect immunofluorescent assay. Measles virus isolation and plaque neutralisation assays weren’t suitable for making routine diagnosis.

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