Expression of LIN9 gene in cervical cancer cells and its relationship with E7 expression of human papilloma virus 16/18

doi:10.3877/cma.j.issn.1674-1358.2021.03.009

Abstract

Abstract:

Objective

To investigate the expression of LIN9 gene in human cervical cancer cell lines of CasKi and HeLa and its relationship with E7 expression of HPV.

Methods

The expressions of LIN9 gene in cervical cancer cell lines CasKi (HPV16+) and HeLa (HPV18+) were detected by qRT-PCR method. Using transfection plasmid to overexpress HPV16/18E7 in human immortalized epidermal cell line HaCaT (HPV-), and siRNA to silence HPV16E7/18E7 expression in cell lines of CasKi and HeLa. The transfection effect was verified by Western blot, and LIN9 mRNA expression levels in cell lines of HaCaT, Caski and HeLa after silent E7 expression were detected by qRT-PCR.

Results

The expressions of LIN9 in CasKi and HeLa cell lines were significantly higher than that in HaCaT cells (1 ± 0.22) (LIN9 in CasKi: 2.55 ± 0.25; t = 22.63, P = 0.026. LIN9 in HeLa: 2.38 ± 0.17; t = 21.21, P = 0.031) and the expression of LIN9 mRNA was increased significantly than that of control group (1 ± 0.14) when overexpressed HPV16/18E7 in HaCaT cells (HPV16E7: 1.65 ± 0.16; t = 13.12, P = 0.036. HPV18E7: 1.61 ± 0.08, t = 11.21, P = 0.039), while decreased significantly after silencing HPV16E7/18E7 expression in CasKi and HeLa cells [(1 ± 0.07) and (1 ± 0.03)] (HPV16E7: 0.50 ± 0.05; t = 12. 73, P = 0.032. HPV18E7: 0.57 ± 0.11; t = 14.30, P = 0.035).

Conclusions

LIN9 was highly expressed in cervical cancer cells, and the expression level was related to the expression of E7. LIN9 may become an important biological marker for monitoring the early HPV infection of cervical cells and the therapeutic effect of cervical cancer.

Key words: Papillomavirus infection, Uterine cervical neoplasms, Biomarkers, tumor

Kunpeng Wang, Yijie Yuan, Jiaqi Zhu, Tingting Zhang. Expression of LIN9 gene in cervical cancer cells and its relationship with E7 expression of human papilloma virus 16/18[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(03): 196-201.

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