Value of real-time fluorescent quantitative polymerase chain reaction in acquired immunodeficiency syndrome complicated with varicella-zoster virus infection

doi:10.3877/cma.j.issn.1674-1358.2019.04.014

Abstract

Abstract:

Objective

To investigate the diagnostic value of varicella zoster virus (VZV) nucleic acid detection in acquired immunodeficiency syndrome (AIDS) complicated with herpes zoster.

Methods

The blood and herpes fluid of AIDS patients with herpes zoster were collected, the virus DNA was detected, and the serum anti-VZV IgM and T cell subsets were detected, respectively.

Results

A total of 32 patients with AIDS complicated with herpes zoster were included, all patients with VZV DNA positive in herpetic fluid, 28 patients with blood VZV DNA positive; only 7 AIDS patients with serum anti-VZV IgM positive, and the load of VZV DNA in herpetic fluid was significantly higher than that in blood, with significant difference (t = 3.173, P = 0.0032). The positive rate of VZV DNA in blood and herpetic fluid were significantly higher than that in serum anti-VZV IgM, with significant difference (87.5%, 100% vs. 21.8%; P < 0.001).

Conclusion

VZV nucleic acid detection was of great value in the etiological diagnosis of AIDS complicated with varicella zoster virus infection.

Key words: Acquired immune deficiency syndrome, Varicella-zoster virus, Real-time quantitative polymerase chain reaction

Wei Zhang, Chengjie Ma, Jian Zhang, Xincheng Song, Yuanni Wu, Rui Zhang, Xin Li. Value of real-time fluorescent quantitative polymerase chain reaction in acquired immunodeficiency syndrome complicated with varicella-zoster virus infection[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(04): 340-343.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsyhlcgrbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1358.2019.04.014

https://zhsyhlcgrbzz.cma-cmc.com.cn/EN/Y2019/V13/I04/340

Figures/Tables 1

References 13

[1]	杜健群, 卢斯汉, 郭庆. 艾滋病合并带状疱疹138例临床分析[J]. 广东医学,2014,35(3):423-425.
[2]	商婷, 彭明霞, 张燕. 以顿挫型带状疱疹为首诊的艾滋病1例[J]. 中国中西医结合皮肤性病学杂志,2017,16(1):76-77.
[3]	Birlea M, Arendt G, Orhan E, et al. Subclinical reactivation of varicella zoster virus in all stages of HIV infection[J]. J Neurol Sci,2011,304(1-2):22-24.
[4]	中华医学会感染病学分会艾滋病学组.艾滋病诊疗指南第三版(2015版)[J]. 中华临床感染病杂志,2015,8(5):385-401.
[5]	赵辨主编. 临床皮肤病学[M]. 3版. 南京: 江苏科学技术出版社,2001:300-302.
[6]	Steiner I, Kennedy PG, Pachner AR.The neurotropic herpes viruses:herpes simplex and varicella-zoster[J]. Lancet Neurol,2007,6(11):1015-1028.
[7]	Lee YC, Hung CC, Tsai MS, et al. Incidence and risk factors of herpes zoster in human immunodeficiency virus-positive patients initiating combination antiretroviral therapy in Taiwan[J]. J Microbiol Immunol Infect,2018,51(1):38-44
[8]	Vora RV, Anjaneyan G, Kota RKS, et al. Study of clinical profile of herpes zoster in human immunodeficiency virus positive and negative patients at a rural-based tertiary care center[J]. Indian J Sex Transm Dis,2017,38(1):65-68.
[9]	卢珍玲, 王官清, 程波. 水痘-带状疱疹病毒感染实验检测方法的研究进展[J]. 国际病毒学杂志,2010,17(1):15-18.
[10]	Engelmann I, Petzold DR, Kosinska A, et al. Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens[J]. J Med Virol,2008,80(3):467-477.
[11]	Weinberg A, Wiznia AA, LaFleur BJ, et al. Varicella-zoster virus-specific cell-mediated immunity in HIV-infected children receiving highly active antiretroviral therapy[J]. J Infect Dis,2004,190(2):267-270.
[12]	L’Huillier AG, Ferry T, Courvoisier DS, et al. Impaired antibody memory to varicella zoster virus in HIV-infected children: low antibody levels and avidity[J]. HIV Med,2012,13(1):54-61.
[13]	De Castro N, Carmagnat M, Kernéis S, et al. Varicella-zoster virus-specific cell-mediated immune responses in HIV-infected adults[J]. AIDS Res Hum Retroviruses,2011,27(10):1089-1097.

指标	阴性	阳性
血清抗-VZV IgM	25（78.1）	7（21.8）
血液VZV DNA	4（12.5）	28（87.5）
疱疹液VZV DNA	0（0.0）	32（100.0）

指标	阴性	阳性
血清抗-VZV IgM	25（78.1）	7（21.8）
血液VZV DNA	4（12.5）	28（87.5）
疱疹液VZV DNA	0（0.0）	32（100.0）

[1]	Yujing Li, Qiyi Chen, Ruming Xie, Budong Chen. Prognostic study of acquired immune deficiency syndrome-related primary central nervous system lymphoma [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(03): 200-208.
[2]	Qian LI, Liping Deng, Guo Chen, Zhongwei Zhang, Pingzheng Mo, Wenjia Hu, Liangjun Chen, Jie Zhang, Yongxi Zhang, Rongrong Yang, Yong Xiong. Clinical application of metagenomic next-generation sequencing in patients with acquired immune deficiency syndrome complicated with central nervous system infection [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(01): 24-31.
[3]	Yanfen Tang, Wen Zhao, Chengjie Ma, Gang Liu, Qi Chen, Jing Liu, Tianjiao Xue, Yanyan Liu, Rongqian Chen, Yu Wang. Clinical characteristics of Mycobacteria avium complex disease in patients with human immunodeficiency virus infection [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(05): 348-353.
[4]	Yu Dong, Yuehong Liu, Xuejing Xu, Binbin Liu. Advantages of ultra-wide-angle laser scanning ophthalmoscope in screening of fundus diseases of patients with acquired immune deficiency syndrome [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(05): 344-347.
[5]	Chunbo Wei, Gang Wan, Dongmei Xu, Xingyun Zhao, Liufeng Yuan, Yan Wu, Wenhui Lun. Clinical and laboratory characteristics of neurosyphilis in 60 patients with human immunodeficiency virus infection/acquired immune deficiency syndrome [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(04): 254-260.
[6]	Yingying Zhou, Liping Deng, Zhiyan Lu, Zhiyin Su, Yong Xiong. A case of acquired immune deficiency syndrome combined with neurosyphilis and cytomegalovirus encephalitis and related literature review [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(05): 350-355.
[7]	Dan Lu, Feifei Mao, Dan Li, Shengnan Wang, Xiyao Liu, Huiyu Sun. Optical coherence tomography manifestations of the acute papilledema in patients with acquired immunodeficiency syndrome complicated with cryptococcal meningitis [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(04): 270-275.
[8]	Naxin Zhao, Zhili Zeng, Guiju Gao, Hongyuan Liang, Jiang Xiao, Di Yang, Fang Wang, Meiling Chen, Jiyun Sun. Value of combined detection of serum cystatin C and urinary microalbumin on detection of human immunodeficiency virus-infected patients with renal dysfunction [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2020, 14(06): 461-466.
[9]	Chunjing Du, Liuluan Zhu, Jingyuan Liu, Ang Li. New advances on the host immune response to Pneumocystis carinii [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2020, 14(06): 447-452.
[10]	Lisong Zhang, Hongxin Zhao, Gaoli Fang, Jing Hou, Aimin Ma. Analysis on laryngopharyngeal lesions of patients with acquired immune deficiency syndrome [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(03): 196-201.
[11]	Rui Ma, Qiang Zhang. Progress of subclinical fracture of patients with acquired immune deficiency syndrome [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(03): 181-184.
[12]	Qiyi Chen, Yunliang Xu, Zhibin Lv, Ruming Xie, Hongxin Zhao, Budong Chen. Comparative study of imaging and pathology of acquired immune deficiency syndrome-related lymphoma [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2018, 12(06): 526-532.
[13]	Wenjun Kong, Yong Tao, Chao Chen, Lianyong Xie, Kuifang Du. Comparison of prognosis of cytomegalovirus retinitis induced by AIDS and HSCT postoperation [J]. Chinese Journal of Ophthalmologic Medicine(Electronic Edition), 2020, 10(01): 13-19.
[14]	Shan Huang, Songqin Lyu, Juan Zhang, Liping Xu, Jianeng Li, Xiaofei Li. The preliminary research on the distribution characteristics of peripheral T lymphocytes subsets in new AIDS patients combined with other pathogenic microorganisms in Yunnan areas [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(01): 16-20.
[15]	Tao Ma, Min Zhang, Peng Tao, Yunhua Chen, Hongmin Sun, Min He, Junjun Wang, Chen LI, Songning Ding, Jie Ding, Dan Lin, Guanghong Xing. Varicella outbreak in a kindergarten and protective effect of the vaccine [J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2020, 06(06): 360-363.

Please choose a citation manager

Content to export