Correlation of human papillomavirus infection among Th17 cell, Treg cell and related cytokines in patients with cervical intraepithelial neoplasia

doi:10.3877/cma.j.issn.1674-1358.2019.04.004

Abstract

Abstract:

Objective

To investigate the correlation of human papillomavirus (HPV) infection with Th17 cell, Treg cell and related cytokines in patients with cervical intraepithelial neoplasia (CIN).

Methods

Total of 140 patients with CIN in Xinjiang Autonomous Region People’s Hospital from June 2017 and May 2018 were selected and divided into infection group (86 cases) and non-infection group (54 cases), according to whether with HPV infection. The expression of CD3, CD4 and CD8 were detected by immunochemistry methods. The T cells and T cell subsets (the percentage of CD3⁺ T cell, CD4⁺ T cell and CD8⁺ T cell), the percentage of Treg cell and Th17 cell (CD4⁺CD25⁺ Treg,CD8⁺CD25⁺CD127^— Treg, CD4⁺Foxp3⁺ Treg, CD8⁺CD25⁺Foxp3⁺ Treg, CD4⁺IL17⁺ Th17 and Th17/Treg) and related cytokines [transforming growth factor-β (TGF-β), IL-10, IL-17 and interferon (IFN)-γ] in the peripheral blood were compared between the two groups. The risk factors of HPV infection in CIN patients analyzed by multivariate Logistic regression model.

Results

No significant difference were found in the expression of CD3, CD4 and CD8 between the two groups. No significant difference were found in CD3⁺ T cell, CD4⁺ T cell and CD8⁺ T cell (t = 0.870, 1.509, 0.236; P = 0.385, 0.133, 0.813). The level of CD4⁺CD25⁺ Treg, CD8⁺CD25⁺CD127^—Treg, CD4⁺Foxp3⁺ Treg, CD8⁺CD25⁺Foxp3⁺ Treg, CD4⁺IL17⁺ Th17 and Th17/Treg in infection group were significantly higher than those of non-infection group (all P < 0.05). The levels of TGF-β, IL-10 and IL-17 were significantly higher in infection group than those of non-infection group (t =-11.601, -42.251, -40.31; all P < 0.001); but the level of IFN-γ was significantly lower in infection group than that of non-infection group (t = 10.316, P < 0.001). Multivariate Logistic regression models showed that CD4⁺CD25⁺ Treg (OR = 3.673, P = 0.031), CD4⁺IL17⁺ Th17 (OR = 1.974, P = 0.021) and Th17/Treg (OR = 3.585, P = 0.024) were all risk factors for HPV infection for patients with CIN. IFN-γ was a protective factor for HPV infection for patients with CIN (OR = 2.812, P = 0.028).

Conclusions

There were correlation between HPV infection and Th17 cell, Treg cell and IFN-γ of CIN patients. CIN patients with HPV infection had increased Th17 cell and Treg cell and decreased IFN-γ.

Key words: Cervical intraepithelial neoplasia, Human papillomavirus, Th17 cell, Treg cell, Cytokine

Zhen Jiao, Juanjuan Fu, Lin Wang. Correlation of human papillomavirus infection among Th17 cell, Treg cell and related cytokines in patients with cervical intraepithelial neoplasia[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(04): 281-286.

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Figures/Tables 4

References 23

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一般资料		非感染组（54例）	感染组（86例）	统计量	P值
年龄（ ± s，岁）		45.26 ± 5.32	44.75 ± 5.34	t = 0.550	0.582
BMI（ ± s，kg/m²）		21.42 ± 2.63	21.79 ± 2.19	t =－0.899	0.369
孕次（ ± s，次）		1.45 ± 0.56	1.51 ± 0.58	t =－0.603	0.547
并发症[例（%）]		?	?	?	?
?	急性感染	0（0.00）	0（0.00）	χ² = 0.044	0.833
?	慢性感染	6（11.11）	8（9.30）	χ² = 0.003	0.953
?	高血压	4（7.40）	7（8.14）	χ² = 0.027	0.868
?	糖尿病	4（7.41）	8（9.30）	χ² = 0.006	0.936
?	心血管疾病	6（11.11）	9（10.46）	χ² = 0.025	0.872
?	其他系统肿瘤	2（3.70）	4（4.65）	χ² = 0.025	0.873
既往史[例（%）]		?	?	?	?
?	吸烟史	1（1.85）	3（3.49）	χ² = 0.001	0.964
?	饮酒史	7（12.96）	13（15.11）	χ² = 0.011	0.915
?	宫颈手术史	5（9.26）	8（9.30）	χ² = 0.084	0.771

一般资料		非感染组（54例）	感染组（86例）	统计量	P值
年龄（ ± s，岁）		45.26 ± 5.32	44.75 ± 5.34	t = 0.550	0.582
BMI（ ± s，kg/m²）		21.42 ± 2.63	21.79 ± 2.19	t =－0.899	0.369
孕次（ ± s，次）		1.45 ± 0.56	1.51 ± 0.58	t =－0.603	0.547
并发症[例（%）]		?	?	?	?
?	急性感染	0（0.00）	0（0.00）	χ² = 0.044	0.833
?	慢性感染	6（11.11）	8（9.30）	χ² = 0.003	0.953
?	高血压	4（7.40）	7（8.14）	χ² = 0.027	0.868
?	糖尿病	4（7.41）	8（9.30）	χ² = 0.006	0.936
?	心血管疾病	6（11.11）	9（10.46）	χ² = 0.025	0.872
?	其他系统肿瘤	2（3.70）	4（4.65）	χ² = 0.025	0.873
既往史[例（%）]		?	?	?	?
?	吸烟史	1（1.85）	3（3.49）	χ² = 0.001	0.964
?	饮酒史	7（12.96）	13（15.11）	χ² = 0.011	0.915
?	宫颈手术史	5（9.26）	8（9.30）	χ² = 0.084	0.771

组别	例数	CD3⁺ T	CD4⁺ T	CD8⁺ T	CD4⁺CD25⁺	CD8⁺CD25⁺ CD127^－ Treg
感染组	86	53.11 ± 13.34	43.23 ± 9.89	30.35 ± 8.34	13.11 ± 2.34	2.55 ± 1.34
非感染组	54	55.23 ± 14.56	45.56 ± 8.21	30.69 ± 8.23	6.23 ± 2.56	1.69 ± 1.23
t值	?	0.870	1.509	0.236	－15.991	－1.621
P值	?	0.385	0.133	0.813	< 0.001	< 0.001
组别	例数	CD4⁺Foxp3⁺ Treg	CD8⁺CD25⁺Foxp3⁺ Treg	CD4⁺IL17⁺ Th17	Th17/Treg
感染组	86	6.33 ± 1.36	0.75 ± 0.21	3.35 ± 1.38	0.25 ± 0.11
非感染组	54	2.67 ± 1.41	0.29 ± 0.12	0.97 ± 0.61	0.12 ± 0.06
t值	?	－15.154	－16.492	－13.985	－9.036
P值	?	< 0.001	< 0.001	< 0.001	< 0.001

组别	例数	CD3⁺ T	CD4⁺ T	CD8⁺ T	CD4⁺CD25⁺	CD8⁺CD25⁺ CD127^－ Treg
感染组	86	53.11 ± 13.34	43.23 ± 9.89	30.35 ± 8.34	13.11 ± 2.34	2.55 ± 1.34
非感染组	54	55.23 ± 14.56	45.56 ± 8.21	30.69 ± 8.23	6.23 ± 2.56	1.69 ± 1.23
t值	?	0.870	1.509	0.236	－15.991	－1.621
P值	?	0.385	0.133	0.813	< 0.001	< 0.001
组别	例数	CD4⁺Foxp3⁺ Treg	CD8⁺CD25⁺Foxp3⁺ Treg	CD4⁺IL17⁺ Th17	Th17/Treg
感染组	86	6.33 ± 1.36	0.75 ± 0.21	3.35 ± 1.38	0.25 ± 0.11
非感染组	54	2.67 ± 1.41	0.29 ± 0.12	0.97 ± 0.61	0.12 ± 0.06
t值	?	－15.154	－16.492	－13.985	－9.036
P值	?	< 0.001	< 0.001	< 0.001	< 0.001

组别	例数	TGF-β	IFN-γ	IL-10	IL-17
感染组	86	45 781.67 ± 14 276.12	8.24 ± 4.12	121.71 ± 17.86	176.81 ± 19.45
非感染组	54	22 360.45 ± 9 612.87	15.23 ± 3.76	24.67 ± 9.23	61.54 ± 17.35
t值	?	－11.601	10.316	－42.251	－40.310
P值	?	< 0.001	< 0.001	< 0.001	< 0.001

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