Inhibitory effect of human umbilical cord mesenchymal stem cells (hUCMSCs) against Pseudomonas aeruginosa

doi:10.3877/cma.j.issn.1674-1358.2018.01.019

Abstract

Abstract:

Objective

To investigate inhibitory effect of the human umbilical cord mesenchymal stem cells (hUCMSCs) on the growth of imipenem-resistant Pseudomonas aeruginosa (IRPA).

Methods

IRPA added into hUCMSCs were collected as the experimental group; while the IRPA co-cultured with fibroblast were taken as the control group. After 6 hours of co-cultivation, colony forming units (CFUs) of IRPA were counted in each group using plate counting method. Then the levels of Cathelicidin/LL-37 and human β-defensin 2 (HBD-2) in cultured medium were detected by Western-blot and ELISA.

Results

The CFUs of IRPA in experimental group were less than those in control groups, with significant differences (2.63 ± 0.58) × 10⁶ CFU/ml vs. (3.69 ± 0.91) × 10⁶ CFU/ml; t = 19.93, P < 0.05). Cathelicidin/LL-37 and hBD2 in experimental group were higher than those of experimental group [Cathelicidin/LL-37: (7.99 ± 0.45) ng/ml vs. (0.18 ± 0.04) ng/ml; t = 78.30, P = 0.007; HBD-2: (249.38 ± 14.19) pg/ml vs. (1.00 ± 0.58) pg/ml; t = 78.23, P = 0.009].

Conclusions

hUCMSCs plays an inhibitory role on the growth of IRPA. The possible mechanism is that hUCMSCs could produce antimicrobial peptide: Cathelicidin/LL-37 and hBD2.

Key words: Human umbilical cord mesenchymal stem cells, Imipenem-resistant Pseudomonas aeruginosa, Cathelicidin/LL-37, Human β-defensin 2

Xiaohong Liu, Haoming Yang, Xuaner Zheng, Shumei Yang, Jie Yang. Inhibitory effect of human umbilical cord mesenchymal stem cells (hUCMSCs) against Pseudomonas aeruginosa[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2018, 12(01): 94-97.

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