Etiology analysis of patients with hand, foot and mouth disease from 2013 to 2015 in a hospital

doi:10.3877/cma.j.issn.1674-1358.2016.06.004

Abstract

Abstract:

Objective

To investigate the pathogens types and distribution features among pediatric patients with hand, foot and mouth diseases (HFMD) in Beijing Ditan Hospital, Peking University Teaching Hospital, from 2013 to 2015, and to provide crucial guidance for the diagnosis, treatment and prevention of HFMD.

Methods

The throat swabs of 84 cases hospitalized in division of pediatrics in our Hospital in 2015 were collected. Real-time fluorescence quantitative (real time-PCR) kits with universal enterovirus (EV) primers, Coxsackievirus A16 (CoxA16)-specifi primers and enterovirus 71 (EV71)-specifi primers were used to detect the samples after RNA extraction. The etiology results of HFMD of 2015 were compared with 2013 and 2014 in our hospital, repectively.

Results

In 2015, the positive rate of EV was 86.9% (73/84), the EV71 accounted for 44.05% (37/84) and the non-EV71, non-CoxA16 enteroviruses accounted for 40.48% (34/84), the CoxA16 enteroviruses accounted for 2.38% (2/84). Compared with 2013, the infection rate of non-EV71, non CoxA16 enteroviruses decreased significantly in 2015 (χ² = 10.20, P = 0.001); the infection rate of EV71 enteroviruses increased significantly (χ² = 28.38, P < 0.001). Compared with 2014, the infection rate of non-EV71 and non-CoxA16 enteroviruses increased significantly in 2015, with extremely significant difference (χ² = 15.50, P < 0.001); the infection rate of EV71 enteroviruses decreased, with significant difference (χ² = 1.89, P = 0.019).

Conclusions

From 2013 to 2015, the changes of distribution and variation of pathogens of HFMD were significant. In 2013, the etiology of pediatric patients with HFMD in our hospital was dominated by EV71 enteroviruses; but in 2014, the EV71 prevailed again. In 2015, the etiology was dominated by EV71, non-EV71 and non-CoxA16 enteroviruses. In prevention and control of HFMD, the non-EV71 and non-CoxA16 enteroviruses infection and EV71 enteroviruses infection should be considered.

Key words: Hand, mouth and foot disease (HFMD), Etiology, Enterovirus

Ting Liu, Hongping Lu, Lin Pang, Yixing Meng, Jinfeng Cao, Yonghong Yan, Ming Han, Shun’ai Liu, Jun Cheng. Etiology analysis of patients with hand, foot and mouth disease from 2013 to 2015 in a hospital[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(06): 663-668.

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Figures/Tables 5

References 20

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组别		例数	EV（+）	EV71（+）	CoxA16（+）	非EV71非CoxA16（+）	EV（-）
年龄（岁）
	0～1	52	43（82.69）	11（21.15）^a	3（5.77）	29（55.77）^b	9（17.31）
	1～2	123	101（82.11）	45（36.59）	10（8.13）	46（37.40）	22（17.89）
	2～3	59	50（84.75）	24（40.68）	3（5.08）	23（38.98）	9（15.25）
	≥ 3	82	65（79.27）	34（41.46）	5（6.10）	28（34.15）	17（20.73）
性别
	男	208	170（81.73）	75（36.06）	14（6.73）	81（38.94）	38（18.27）
	女	108	89（82.41）	37（34.26）	7（6.48）	45（41.67）	19（17.59）

组别		例数	EV（+）	EV71（+）	CoxA16（+）	非EV71非CoxA16（+）	EV（-）
年龄（岁）
	0～1	52	43（82.69）	11（21.15）^a	3（5.77）	29（55.77）^b	9（17.31）
	1～2	123	101（82.11）	45（36.59）	10（8.13）	46（37.40）	22（17.89）
	2～3	59	50（84.75）	24（40.68）	3（5.08）	23（38.98）	9（15.25）
	≥ 3	82	65（79.27）	34（41.46）	5（6.10）	28（34.15）	17（20.73）
性别
	男	208	170（81.73）	75（36.06）	14（6.73）	81（38.94）	38（18.27）
	女	108	89（82.41）	37（34.26）	7（6.48）	45（41.67）	19（17.59）

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