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中华实验和临床感染病杂志(电子版) ›› 2016, Vol. 10 ›› Issue (05) : 559 -564. doi: 10.3877/cma.j.issn.1674-1358.2016.05.010

临床论著

腹腔感染大肠埃希菌PFGE谱型及耐药一致性分析
李玉芝1, 杜小丽2, 陈志海1,()   
  1. 1. 100015 北京,首都医科大学附属北京地坛医院感染二科
    2. 102206 北京,中国疾病预防控制中心传染病预防控制所
  • 收稿日期:2015-07-27 出版日期:2016-10-15
  • 通信作者: 陈志海
  • 基金资助:
    北京市卫生系统高层次卫生技术人才培养计划(No. 2011-3-079)

PFGE analysis and antimicrobial resistance of E. coli isolated from intra-abdominal infection

Yuzhi Li1, Xiaoli Du2, Zhihai Chen1,()   

  1. 1. Department of Infectious Diseases, Division 2ed, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
    2. Pulse Net China, Chinese Center for Diseases Control and Prevention, Beijing 102206, China
  • Received:2015-07-27 Published:2016-10-15
  • Corresponding author: Zhihai Chen
引用本文:

李玉芝, 杜小丽, 陈志海. 腹腔感染大肠埃希菌PFGE谱型及耐药一致性分析[J/OL]. 中华实验和临床感染病杂志(电子版), 2016, 10(05): 559-564.

Yuzhi Li, Xiaoli Du, Zhihai Chen. PFGE analysis and antimicrobial resistance of E. coli isolated from intra-abdominal infection[J/OL]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(05): 559-564.

目的

探究腹腔感染大肠埃希菌菌株间的相关性及菌株间同源性与药敏结果一致性的关系。

方法

使用限制性内切酶XbaⅠ对菌株基因组进行酶切,对酶切后的DNA片段进行脉冲场凝胶电泳( PFGE),使用Bionumerics软件分析菌株间相关性,将菌株同源性与药敏结果一致性进行对比分析。

结果

47株大肠埃希菌PFGE图谱间同源性为60.4%~97.4%,同源性高于85%有6株。一次自发性细菌性腹膜炎(SBP)可以由一种PFGE谱型的大肠埃希菌引起,也可能是多种PFGE谱型大肠埃希菌的混合感染。一次SBP可能是新发感染,也可能是上次感染的复发。同源性高于85%的菌株对本研究中19种抗菌药物中的15种以上抗菌药物的药敏试验结果相同。

结论

导致不同患者腹腔感染的大肠埃希菌来源不同。同源性高于85%的菌株药敏结果具有较高的一致性。

Objective

To study the relationship of E.coli strains isolated from intra-abdominal infection, and the relationship of homology and the antimicrobial susceptibility results of different strains.

Methods

DNA of strains were digested with XbaⅠ restriction enzyme, then were typed by pulsed field gel electrophoresis (PFGE). PFGE patterns were analyzed with Bionumerrics software. The antimicrobial susceptibility results of E. coli strains and the clinical information of patients were collected.

Results

The homology of strains were from 60.4% to 97.4%, 6 among 47 strains were above 85%. One episode of SBP could be a result of reinfection caused by one PFGE type strain, or more than one PFGE type strains, also could be a recurrent infection of the last episode. Antimicrobial susceptibility results of more than 15 antibiotics were indentical among strains with genetic similarity ≥ 85%.

Conclusions

Most strains isolated from intra-abdominal infection were different. Strains with genetic similarity ≥ 85% have identical or similar antimicrobial susceptibility .

图1 菌株PFGE图谱-1
图2 菌株PFGE图谱-2
图3 菌株PFGE图谱-3
图4 菌株PFGE图谱-4
图5 菌株PFGE图谱-5
图6 大肠埃希菌PFGE谱型聚类分析图
图7 相同患者多次分离菌株PFGE图谱聚类分析结果(同一登记号为同一患者)
1
Talon D, Cailleaux V, Thouverez M, et al. Discriminatory power and usefulness of pulsed-field gel electrophoresis in epidemiological studies of Pseudomonas aeruginosa [J]. J Hosp Infect,1996,32(2):135-145.
2
Hunter SB, Vauterin P, Lambert-Fair MA, et al. Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard[J]. J Clin Microbiol,2005,43(3):1045-1050.
3
李伟,崔志刚,阚飙, 等. 转化预防医学的实践: PulseNet China[J]. 疾病监测,2012,27(2):88-92.
4
Centers for Disease Control and Prevention (CDC). Multistate outbreak of campylobacter jejuni infections associated with undercooked chicken livers-Northeastern United States, 2012[J]. MMWR,2013,62(44):874-876.
5
Centers for Disease Control and Prevention (CDC. Multistate outbreak of salmonella chester infections associated with frozen meals-18 States, 2010[J]. MMWR,2013,62(48):979-982.
6
Centers for Disease Control and Prevention (CDC). Outbreak of Salmonella Heidelberg infections linked to a single poultry producer-13 states, 2012-2013[J]. MMWR,2013,62(27):553-556.
7
Centers for Disease Control and Prevention (CDC. Outbreak of invasive listeriosis associated with the consumption of hog head cheese--Louisiana, 2010[J]. MMWR,2011,60(13):401.
8
Gaulin C, Ramsay D, Bekal S. Widespread listeriosis outbreak attributable to pasteurized cheese, which led to extensive cross-contamination affecting cheese retailers, Quebec, Canada, 2008[J]. J Food Prot,2012,75(1):71-78.
9
赵萌,崔旭初,于慧霞, 等. 一起沙门菌食物中毒的实验室诊断与分析[J]. 疾病监测,2015,30(5):381-384.
10
Fernández CF, López CL, Pascual HA. Molecular typing methods for infection monitoring and control[J]. Enferm Infecc Microbiol Clin,2013,31(Suppl 1):20-25.
11
Seki M, Machida N, Yamagishi Y, et al. Nosocomial outbreak of multidrug-resistant Pseudomonas aeruginosa caused by damaged transesophageal echocardiogram probe used in cardiovascular surgical operations[J]. J Infect Chemother,2013,19(4):677-681.
12
Kiddee A, Henghiranyawong K, Yimsabai J, et al. Nosocomial spread of class 1 integron-carrying extensively drug-resistant Pseudomonas aeruginosa isolates in a Thai hospital[J]. Int J Antimicrob Agents,2013,42(4):301-306.
13
代文霞,沈叙庄,高薇, 等. 耐甲氧西林金黄色葡萄球菌引起医院内感染的基因分型及耐药监测[J]. 中华流行病学杂志,2009,29(7):740-741.
14
宣迎梅. 院内感染多重耐药鲍曼不动杆菌的同源性分析[J]. 吉林医学,2012,33(34):7434-7436.
15
Yu F, Ying Q, Chen C, et al. Outbreak of pulmonary infection caused by Klebsiella pneumoniae isolates harbouring blaIMP-4 and blaDHA-1 in a neonatal intensive care unit in China[J]. J Medical Microbiol,2012,61(Pt 7):984-989.
16
Sun H, Wang H, Xu Y, et al. Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China[J]. Diagn Microbiol Infect Dis,2012,74(4):399-403.
17
Luo Y, Ma Y, Zhao Q, et al. Similarity and divergence of phylogenies, antimicrobial susceptibilities, and virulence factor profiles of Escherichia coli isolates causing recurrent urinary tract infections that persist or result from reinfection[J]. J Clin Microbiol,2012,50(12):4002-4007.
18
代文霞,沈叙庄,高薇, 等. 耐甲氧西林金黄色葡萄球菌引起医院内感染的基因分型及耐药监测[J]. 中华流行病学杂志,2008,29(7):740-741.
19
王红,赵爱兰,白向宁, 等. 一起肠产毒性大肠杆菌O128: H45引起的院内新生儿腹泻暴发[J]. 中国人兽共患病学报,2013,29(3):304-308.
20
Yu F, Ying Q, Chen C, et al. Outbreak of pulmonary infection caused by Klebsiella pneumoniae isolates harbouring blaIMP-4 and blaDHA-1 in a neonatal intensive care unit in China[J]. J Med Microbiol,2012,61(Pt 7):984-989.
21
宣迎梅. 院内感染多重耐药鲍曼不动杆菌的同源性分析[J]. 吉林医学,2012,33(34):7434-7436.
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