Expression of interleukin-6 and phosphorylated signal transduction and activator of transcription 3 in pregnant women with group B Streptococcus positive

doi:10.3877/cma.j.issn.1674-1358.2020.03.012

Abstract

Abstract:

Objective

To investigate the expression of interleukin 6 (IL-6) and phosphorylation signal transduction and transcription activation factor 3 (p-STAT3) in B group of Streptococcus (GBS)-positive preterm pregnant women and the relationship with preterm birth.

Methods

From June 2018 to June 2019, a total of 1 950 pregnant women were selected from the outpatient antenatal examination to Maternal and Child Health Hospital, Futian District waiting for delivery, among whom, 122 cases with GBS positive. Vaginal secretions from 20 cases with GBS positive premature delivery (premature delivery group), 33 cases with full-term premature rupture of membranes (premature rupture of membranes), 66 cases with full-term vaginal delivery (full-term delivery group), 3 cases with abortion (abortion group) and 20 cases with GBS negative (GBS negative control group) were suspended, the concentrations of IL-6 were detected by ELISA method, the expressions of p-STAT3, p-Akt and NF-κB subunit p65 were detected by Western blot.

Results

The GBS positive rate of pregnant women was 6.25% (122/1 950), among whom, abortion, premature delivery, full- term premature rupture of membranes and full-term delivery accounted for 2.45% (3/122), 16.12% (20/122), 26.62% (33/122) and 53.66% (66/122), respectively. The expression of IL-6 in GBS positive premature delivery group [(0.065 ± 0.034) pg/ml] was significantly higher than that of full-term delivery group [(0.045 ± 0.021) pg/ml] (t =-3.192, P = 0.002) and GBS negative control group [(0.043 ± 0.020) pg/ml] (t =-2.494, P = 0.017), both with significant differences. p-STAT3 expressions in GBS positive premature delivery group (gray value: 0.06 ± 0.03) was significantly higher than that of GBS negative control group (gray value: 0.04 ± 0.01), with significant difference (t =-2.9812, P = 0.005); The gray values of p-Akt and p65 in GBS positive premature delivery group and GBS negative control group [p-Akt: (0.035 ± 0.02) vs. (0.07 ± 0.03), p65: (0.045 ± 0.02) vs. (0.085 ± 0.04)] were with significant differences (t = 4.341, P = 0.001; t = 4.000, P = 0.003).

Conclusions

Preterm delivery in GBS positive pregnant women may be closely related to high expression of IL-6, p-STAT3, p-Akt and p65 in vaginal discharge.

Key words: Group B Streptococcus, Premature delivery, Interleukin-6, Phosphorylated signal transduction and activator of transcription 3, Premature rupture of membranes.

Hui Yi, Yunxia Wang, Huifang He. Expression of interleukin-6 and phosphorylated signal transduction and activator of transcription 3 in pregnant women with group B Streptococcus positive[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2020, 14(03): 247-252.

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References 27

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组别	例数	年龄（ ± s，岁）	BMI（ ± s，Kg/m²）	孕周（ ± s，周）	合并症种数（ ± s，种）	产次（ ± s，次）
早产组	20	29.58 ± 5.79	22.75 ± 2.71	10.0 ± 1.5	1.72 ± 1.01	1.83 ± 0.93
胎膜早破组	33	28.98 ± 4.92	22.82 ± 2.83	9.8 ± 1.3	2.33 ± 2.34	2.03 ± 1.02
足月分娩组	66	29.50 ± 5.77	22.28 ± 3.11	9.6 ± 1.1	2.77 ± 2.36	1.95 ± 0.85
流产组	3	29.45 ± 5.75	23.02 ± 3.12	9.5 ± 1.1	2.18 ± 2.08	2.02 ± 1.02
GBS阴性对照组	20	28.95 ± 5.12	23.01 ± 3.01	9.7 ± 1.2	1.17 ± 1.02	2.01 ± 1.05
F值	?	0.083	0.357	0.480	2.744	0.162
P值	?	0.987	0.839	0.750	0.510	0.957

组别	例数	年龄（ ± s，岁）	BMI（ ± s，Kg/m²）	孕周（ ± s，周）	合并症种数（ ± s，种）	产次（ ± s，次）
早产组	20	29.58 ± 5.79	22.75 ± 2.71	10.0 ± 1.5	1.72 ± 1.01	1.83 ± 0.93
胎膜早破组	33	28.98 ± 4.92	22.82 ± 2.83	9.8 ± 1.3	2.33 ± 2.34	2.03 ± 1.02
足月分娩组	66	29.50 ± 5.77	22.28 ± 3.11	9.6 ± 1.1	2.77 ± 2.36	1.95 ± 0.85
流产组	3	29.45 ± 5.75	23.02 ± 3.12	9.5 ± 1.1	2.18 ± 2.08	2.02 ± 1.02
GBS阴性对照组	20	28.95 ± 5.12	23.01 ± 3.01	9.7 ± 1.2	1.17 ± 1.02	2.01 ± 1.05
F值	?	0.083	0.357	0.480	2.744	0.162
P值	?	0.987	0.839	0.750	0.510	0.957

组别	例数	IL-6	^at值	^aP值
早产组	20	0.065 ± 0.03	－2.494	0.017
胎膜早破组	33	0.045 ± 0.02	－0.353	0.726
足月分娩组	66	0.045 ± 0.02	－0.377	0.707
流产组	3	0.059 ± 0.03	－1.214	0.238
GBS阴性对照组	20	0.043 ± 0.02	—	—
F值	?	4.528	?	?
P值	?	0.005	?	?

组别	例数	IL-6	^at值	^aP值
早产组	20	0.065 ± 0.03	－2.494	0.017
胎膜早破组	33	0.045 ± 0.02	－0.353	0.726
足月分娩组	66	0.045 ± 0.02	－0.377	0.707
流产组	3	0.059 ± 0.03	－1.214	0.238
GBS阴性对照组	20	0.043 ± 0.02	—	—
F值	?	4.528	?	?
P值	?	0.005	?	?

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