Influence of male reproductive tract pathogen infection on semen quality and sperm morphology

doi:10.3877/cma.j.issn.1674-1358.2017.03.010

Abstract

Abstract:

Objective

To investigate the influence of pathogen infection on semen quality and sperm morphology, and to provide theoretical knowledge and reference basis for the clinical treatment.

Methods

Semen samples from 658 cases among 2 093 male patients from 2014 to 2015 were detected for Ureaplasma urealyticum (UU), Mycoplasma hominis (MH), Chlamydia trachomatis (CT), Nesisseriagonarrhoeae (NG) and sperm morphology, respectively. The routine semen analysis and seminal morphology parameters were carried out among patients with pathogen infection and without pathogen infection.

Results

No pathogen infection was detected in 543 semen samples among 658 samples and no NG infection was found in all semen samples. The positive rates of UU, MH and CT were 15.81% (104/658), 0.76% (5/658) and 0.45% (3/658), respectively. The double positive rate for UU and MH was 0.76 % (5/658). The pH (F = 4.01, P = 0.003 ), liquefaction time (F = 7.47, P < 0.001 ), sperm density (F = 23.42, P < 0.001), non-progressive motility(F = 37.61, P < 0.001) or progressive motility (F = 26.7, P < 0.001) and sperm activity rates between patients with pathogen infection and without pathogen infection were significantly different. Compared with patients without pathogen infection, the important parameters of sperm morphology including spermatozoa (F = 18.88, P < 0.001), the head deformities (F = 116.23, P < 0.001), the middle piece of deformities (F = 20.47, P < 0.001), the principal piece of deformity (F = 3.22, P = 0.012) and round cells (F = 9.04, P < 0.001) of patients with pathogen infection were significantly different.

Conclusion

Pathogen infection might affect semen quality and sperm morphology.

Key words: Male, Reproductive tract infections, Routine semen analysis, Seminal morphology

Yun Li, Fan Yang, Zhou Junyan se, Qiankun Xuan, Lihua He. Influence of male reproductive tract pathogen infection on semen quality and sperm morphology[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2017, 11(03): 255-259.

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References 25

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参数	参考值下限
精液体积（ml）	1.5（1.4～1.7）
总精子数	39 × 10⁶（33～46）/一次射精
精子浓度	15 × 10⁶（12～16）/ml
总活力（前向运动+非前向运动）	40%（38%～42%）
前向运动	32%（31%～34%）
存活率（活精子）	58%（55%～63%）
精子形态学（正常形态）	4%（3%～4%）
pH值	≥ 7.2

参数	参考值下限
精液体积（ml）	1.5（1.4～1.7）
总精子数	39 × 10⁶（33～46）/一次射精
精子浓度	15 × 10⁶（12～16）/ml
总活力（前向运动+非前向运动）	40%（38%～42%）
前向运动	32%（31%～34%）
存活率（活精子）	58%（55%～63%）
精子形态学（正常形态）	4%（3%～4%）
pH值	≥ 7.2

指标	阴性（-）	UU（+）	MH（+）	UU + MH （+）	CT（+）	F值	P值
年龄（岁）	33.87 ± 6.41	35.05 ± 7.59	35 ± 2.45	36.2 ± 8.47	38.5 ± 7.78	1.06	0.377
量（ml）	3.15 ± 1.58	2.98 ± 1.18	2.59 ± 0.56	2.80 ± 0.82	2.21 ± 0.39	1.59	0.175
pH值	7.22 ± 0.09	7.21 ± 0.10	7.20 ± 0.00	7.20 ± 0.00	7.20 ± 0.00	4.01	0.003
液化时间（min）	30.29 ± 2.72	30.58 ± 4.12	30.00 ± 0.00	30.00 ± 0.00	30.00 ± 0.00	7.47	< 0.001
浓度（× 10⁶/ml）	65.47 ± 39.56	67.52 ± 33.16	84.23 ± 41.86	73.89 ± 25.10	62.69 ± 19.16	23.42	< 0.001
前向（%）	35.55 ± 12.63	36.49 ± 12.71	38.98 ± 6.83	43.32 ± 8.79	18.86 ± 6.55	36.71	< 0.001
非前向（%）	27.62 ± 8.73	28.10 ± 9.25	34.42 ± 9.33	23.65 ± 3.70	19.73 ± 5.19	37.61	< 0.001
不活动（%）	52.35 ± 16.23	49.97 ± 15.16	31.39 ± 6.97	39.89 ± 11.52	71.87 ± 13.89	162.70	< 0.001
活率（%）	58.59 ± 15.12	59.20 ± 13.39	68.80 ± 6.70	65.18 ± 10.02	37.46 ± 12.69	88.78	< 0.001
VAP（μm/s）	13.86 ± 12.87	11.15 ± 4.04	12.03 ± 2.86	42.22 ± 25.16	4.68 ± 1.71	181.81	< 0.001
VCL（μm/s）	12.95 ± 4.51	12.68 ± 4.06	14.23 ± 2.64	16.42 ± 4.27	6.82 ± 2.53	18.96	< 0.001
ALH（μm）	1.28 ± 2.29	0.67 ± 0.19	0.76 ± 0.07	0.75 ± 0.16	0.39 ± 0.00	1.25	0.291
BCF（Hz）	13.02 ± 1.33	12.80 ± 1.03	12.48 ± 0.59	13.08 ± 1.18	13.61 ± 0.35	12.04	< 0.001
MAD（μm/s）	101.93 ± 11.76	99.64 ± 10.62	96.13 ± 7.37	94.23 ± 4.24	111.62 ± 0.82	200.42	< 0.001
VSL（μm/s）	17.35 ± 26.98	7.80 ± 2.80	8.64 ± 2.52	10.58 ± 2.95	3.47 ± 1.34	22.99	< 0.001
直线运动精子活率	39.51 ± 13.51	40.41 ± 13.08	42.99 ± 5.75	47.22 ± 9.13	19.98 ± 6.99	39.24	< 0.001
WOB（%）	67.94 ± 12.80	68.46 ± 12.47	71.62 ± 7.56	76.70 ± 9.26	49.70 ± 2.57	105.20	< 0.001
LIN（%）	44.58 ± 9.74	44.62 ± 9.15	45.69 ± 6.28	51.15 ± 7.04	30.24 ± 2.33	63.47	< 0.001
STR（%）	60.60 ± 6.60	60.52 ± 6.34	60.94 ± 5.96	63.88 ± 4.14	54.75 ± 0.00	42.53	< 0.001
黏稠度						0.738	0.566

指标	阴性（-）	UU（+）	MH（+）	UU + MH （+）	CT（+）	F值	P值
年龄（岁）	33.87 ± 6.41	35.05 ± 7.59	35 ± 2.45	36.2 ± 8.47	38.5 ± 7.78	1.06	0.377
量（ml）	3.15 ± 1.58	2.98 ± 1.18	2.59 ± 0.56	2.80 ± 0.82	2.21 ± 0.39	1.59	0.175
pH值	7.22 ± 0.09	7.21 ± 0.10	7.20 ± 0.00	7.20 ± 0.00	7.20 ± 0.00	4.01	0.003
液化时间（min）	30.29 ± 2.72	30.58 ± 4.12	30.00 ± 0.00	30.00 ± 0.00	30.00 ± 0.00	7.47	< 0.001
浓度（× 10⁶/ml）	65.47 ± 39.56	67.52 ± 33.16	84.23 ± 41.86	73.89 ± 25.10	62.69 ± 19.16	23.42	< 0.001
前向（%）	35.55 ± 12.63	36.49 ± 12.71	38.98 ± 6.83	43.32 ± 8.79	18.86 ± 6.55	36.71	< 0.001
非前向（%）	27.62 ± 8.73	28.10 ± 9.25	34.42 ± 9.33	23.65 ± 3.70	19.73 ± 5.19	37.61	< 0.001
不活动（%）	52.35 ± 16.23	49.97 ± 15.16	31.39 ± 6.97	39.89 ± 11.52	71.87 ± 13.89	162.70	< 0.001
活率（%）	58.59 ± 15.12	59.20 ± 13.39	68.80 ± 6.70	65.18 ± 10.02	37.46 ± 12.69	88.78	< 0.001
VAP（μm/s）	13.86 ± 12.87	11.15 ± 4.04	12.03 ± 2.86	42.22 ± 25.16	4.68 ± 1.71	181.81	< 0.001
VCL（μm/s）	12.95 ± 4.51	12.68 ± 4.06	14.23 ± 2.64	16.42 ± 4.27	6.82 ± 2.53	18.96	< 0.001
ALH（μm）	1.28 ± 2.29	0.67 ± 0.19	0.76 ± 0.07	0.75 ± 0.16	0.39 ± 0.00	1.25	0.291
BCF（Hz）	13.02 ± 1.33	12.80 ± 1.03	12.48 ± 0.59	13.08 ± 1.18	13.61 ± 0.35	12.04	< 0.001
MAD（μm/s）	101.93 ± 11.76	99.64 ± 10.62	96.13 ± 7.37	94.23 ± 4.24	111.62 ± 0.82	200.42	< 0.001
VSL（μm/s）	17.35 ± 26.98	7.80 ± 2.80	8.64 ± 2.52	10.58 ± 2.95	3.47 ± 1.34	22.99	< 0.001
直线运动精子活率	39.51 ± 13.51	40.41 ± 13.08	42.99 ± 5.75	47.22 ± 9.13	19.98 ± 6.99	39.24	< 0.001
WOB（%）	67.94 ± 12.80	68.46 ± 12.47	71.62 ± 7.56	76.70 ± 9.26	49.70 ± 2.57	105.20	< 0.001
LIN（%）	44.58 ± 9.74	44.62 ± 9.15	45.69 ± 6.28	51.15 ± 7.04	30.24 ± 2.33	63.47	< 0.001
STR（%）	60.60 ± 6.60	60.52 ± 6.34	60.94 ± 5.96	63.88 ± 4.14	54.75 ± 0.00	42.53	< 0.001
黏稠度						0.738	0.566

指标（%）	阴性（-）	UU（+）	MH（+）	UU + MH （+）	CT（+）	F值	P值
正常形态	7.33 ± 3.20	7.40 ± 3.24	11.11 ± 3.62	8.98 ± 4.52	6.39 ± 2.04	18.88	< 0.001
头部缺陷	89.48 ± 4.63	89.44 ± 4.65	84.60 ± 4.98	89.66 ± 5.44	89.34 ± 2.76	116.23	< 0.001
中段缺陷	20.05 ± 6.70	21.15 ± 6.84	23.79 ± 4.77	19.94 ± 3.31	17.49 ± 4.30	20.47	< 0.001
主段缺陷	16.18 ± 6.42	16.42 ± 5.31	16.64 ± 3.71	14.66 ± 2.54	13.20 ± 0.20	3.22	0.012
过量胞浆	1.72 ± 2.08	1.39 ± 0.96	0.00 ± 0.00	0.75 ± 0.35	1.00 ± 0.00	0.68	0.563
圆形细胞	0.96 ± 0.91	2.47 ± 2.31	0.00 ± 0.00	0.30 ± 0.00	0.589	9.04	< 0.001

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