Research progress on molecular detection methods for resistance and virulence genes of Helicobacter pylori

doi:10.3877/cma.j.issn.1674-1358.2026.01.003

Abstract

Abstract:

Helicobacter pylori is one of the most important pathogens causing human gastrointestinal diseases and has been classified as a Group 1 carcinogen by the World Health Organization (WHO). Accurate detection of Helicobacter pylori is of great significance for clinical diagnosis and treatment. Currently, a variety of routine clinical diagnostic methods are available, with serological antibody detection, ¹³C/¹⁴C urea breath test (UBT), microaerophilic culture and histological staining being the most commonly used. However, due to the overuse of antibacterial agents, Helicobacter pylori drug resistance has become increasingly prevalent, rendering traditional detection methods inadequate to fully meet the demands of personalized medicine. Molecular detection technologies, which target drug resistance and virulence genes, have emerged as crucial tools urgently required for clinical practice and scientific research, providing novel solutions for clinical management. This review summarizes the research progress of molecular detection methods for Helicobacter pylori, discusses their clinical application value and technical limitations, and aims to provide a theoretical basis for the selection of individualized and precise diagnosis and treatment strategies for Helicobacter pylori infection.

Key words: Helicobacter pylori, Drug resistance genes, Molecular detection, Real-time fluorescent quantitative polymerase chain reaction, Genome sequencing

Qingzhan Lan, Boqing Li, Tong Mu, Zhiming Lu, Yan Jin, Chunhong Shao. Research progress on molecular detection methods for resistance and virulence genes of Helicobacter pylori[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2026, 20(01): 10-14.

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