Diagnostic value of reverse-transcriptase quantitative polymerase chain reaction for Mycobacterium tuberculosis infection based on 85B mRNA

doi:10.3877/cma.j.issn.1674-1358.2019.04.010

Abstract

Abstract:

Objective

To investigate the diagnostic value of reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) targeting 85B mRNA of Mycobacterium tuberculosis (MTB) in sputum samples for MTB infection.

Methods

Total of 60 cases with MTB infection in the experimental group by sputum culture method (BACTEC MGIT 960 system) in the Six Branch of Shanxi Tuberculosis Control Hospital from January 2016 to December 2016 were selected as research group, while 60 healthy people without MTB infection were collected as control group. The nucleic acids were extracted from sputum samples. The DNA sequence encoding 16S rRNA of MTB was detected by TaqMan method and the 85B mRNA of MTB was detected by RT-qPCR method, then the diagnostic efficacy (including sensitivity, specificity, positive predictive value, negative predictive value, and accuracy) of the two methods were compared.

Results

The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of TaqMan method for the diagnosis of patients in research group were 93.3%, 83.3%, 84.9%, 92.6% and 88.3%, respectively; which were 98.3%, 95.0%, 95.2%, 98.3% and 96.7% of RT-PCR, respectively. The binary Logistic regression analysis confirmed that RT-qPCR based on 85B mRNA was more optimal to diagonse MTB infection (OR = 19.924, 95%CI: 5.364-73.012, P < 0.001).

Conclusions

RT-qPCR targeting 85B mRNA of MTB could rapidly and effectively diagnose the status of MTB infection.

Key words: Mycobacterium tuberculosis, Reverse-transcriptase quantitative polymerase chain reaction, Diagnosis

Juxia Zhang, Wanfu Yang. Diagnostic value of reverse-transcriptase quantitative polymerase chain reaction for Mycobacterium tuberculosis infection based on 85B mRNA[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(04): 316-319.

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References 26

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检测方法		试验组（60例）	对照组（60例）	χ²值	P值
MGIT 960法		?	?	120.000	< 0.001
?	阳性	60（100.0）	0（0.0）	?	?
?	阴性	0（0.0）	60（100.0）	?	?
TaqMan法		?	?	71.246	< 0.001
?	阳性	56（93.3）	10（16.7）	?	?
?	阴性	4（6.7）	50（83.3）	?	?
RT-qPCR法		?	?	104.650	< 0.001
?	阳性	59（98.3）	3（5.0）	?	?
?	阴性	1（1.7）	57（95.0）	?	?

检测方法		试验组（60例）	对照组（60例）	χ²值	P值
MGIT 960法		?	?	120.000	< 0.001
?	阳性	60（100.0）	0（0.0）	?	?
?	阴性	0（0.0）	60（100.0）	?	?
TaqMan法		?	?	71.246	< 0.001
?	阳性	56（93.3）	10（16.7）	?	?
?	阴性	4（6.7）	50（83.3）	?	?
RT-qPCR法		?	?	104.650	< 0.001
?	阳性	59（98.3）	3（5.0）	?	?
?	阴性	1（1.7）	57（95.0）	?	?

检测方法	B值	SE值	Wald χ²值	P值	OR值	95%CI
常量	2.473	1.715	2.080	0.149	11.858	0.872～19.242
TaqMan法	1.204	0.720	2.794	0.095	3.332	0.812～13.669
RT-qPCR法	2.992	0.670	19.968	< 0.001	19.924	5.364～74.012

检测方法	B值	SE值	Wald χ²值	P值	OR值	95%CI
常量	2.473	1.715	2.080	0.149	11.858	0.872～19.242
TaqMan法	1.204	0.720	2.794	0.095	3.332	0.812～13.669
RT-qPCR法	2.992	0.670	19.968	< 0.001	19.924	5.364～74.012

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