Mycoplasma hominis is a kind of conditional pathogenic microorganism for human beings. In addition to urogenital mucosal infection and pulmonary infection, the infection of other organs and tissues caused by Mycoplasma hominis had received more and more attention of clinical workers. Nevertheless, Mycoplasma hominis infection usually showed no symptom, atypical clinical symptoms, or diversified symptoms, it was difficult to make correct identification because the conventional cultivation and identification of pathogen in the laboratory were restricted by many factors, and the extensive use of antibacterial drugs would induce multiple drug-resistant strains, thereby making the clinical diagnosis and treatment of these infections more complicated, so it very easy to be neglected or misdiagnosed. Therefore, it was still an exploratory issue for both clinic and laboratory to take early diagnosis and treatment for Mycoplasma hominis infection. In this paper, the characteristics of Mycoplasma hominis infection in other organs and tissues were briefly introduced. Several detection and identification techniques of Mycoplasma hominis and their drug resistance were reviewed in order to provide reference for clinical diagnosis and treatment of Mycoplasma hominis.