Changes of serum Golgi protein 73 of patients with influenza

doi:10.3877/cma.j.issn.1674-1358.2019.03.004

Abstract

Abstract:

Objective

To investigate the changes of serum Golgi protein 73 (G73) of patients with influenza and the effect on the outcome of prognosis.

Methods

From July 2017 to October 2018, a total of 83 patients with influenza (influenza group) who were hospitalized in the 910th Hospital of the People’s Liberation Army were collected, and 50 healthy cases for physical examination were selected as the control group. Serum GP73 concentration was detected by ELISA method. The changes of serum GP73, procalcitonin (PCT) and interleukin-6 (IL6) levels were detected in patients with influenza at the timepoints of admission with fever, after fever and 1 month after discharge from hospital. The levels of serum GP73, PCT and IL-6 were expressed by median (quartile spacing). Variance analysis was applied to compare the differences of the above indexes between patients of the two groups. Wilcoxon test was applied to analyze the changes of each index before and after treatment, and linear regression and Spearman rank correlation coefficient (r) were used to evaluate the correlation between variables.

Results

Total of 72 patients (86.75%) with influenza A virus infection and 11 cases (13.25%) of influenza B virus infection were detected among 83 patients with influenza. Among the 72 cases with influenza A virus infection, 65 cases (90.28%) were H3 subtype and 7 cases (9.72%) were H1 subtype. No other subtypes of influenza virus, such as H5, H7, H9 and so on were detected. The serum GP73 concentration of influenza patients with fever was 137.7 (100.6, 179.4) ng/ml, which was significantly higher than that of control group [32.9 (23.0, 52.25) ng/ml], with significant difference (F = 12.30, P < 0.001). The IL-6 concentration of patients with influenza when admitted to hospital with fever was [12.98 (11.28, 19.80)] ng/ml, significantly higher than that of control group [1.52 (1.31, 1.72) ng/ml], with significant difference (F = 15.61, P < 0.001), and the level of serum PCT was 0.032 (0.022, 0.082) ng/ml, slightly higher than that of control group [0.025 ng/ml (0.019, 0.035) ng/ml], but with no significant difference (F = 1.52, P = 0.253). The serum G73 content of patients with influenza was significantly decreased, and the content of serum G73 was 80.13 (60.20, 96.54) ng/ml and 46.65 (40.47, 55.59) ng/ml after fever and follow-up for 1 month; and compared with the concentration of serum G73 [137.7 (100.6, 179.4) ng/ml] of the patients with fever when admitted to hospital, both with significant differences (afer fever vs. at fever: Z =-9.46, P < 0.001; follow-up for 1 month vs. at fever: Z =-15.23, P < 0.001).

Conclusions

The concentration of serum GP73 of influenza patients with fever increased significantly and decreased rapidly after fever. Serum GP73 may be involved in the process of inflammatory injury and repairment of patients with influenza , which could be taken as a rapid reaction protein for injury or repairment of acute inflammation.

Key words: Influenza virus, Golgi protein 73 (GP73), Clinical characteristics, Prognosis

Zhengju Xu, Houquan Hu, Dandan Xie, Xiaoman Zhang, Liguan Liu, Lianqiu Zheng, Yanyan Yan, Zhijie Huang. Changes of serum Golgi protein 73 of patients with influenza[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(03): 190-195.

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References 24

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流感病毒	引物名称	序列（5'→3'）
A型	上游引物	GAC CRA TCC TGT CAC CTC TGA C
	下游引物	GGG CAT TYT GGA CAA AKC GTC TAC G
	探针	FAM- TGC AGT CCT CGC TCA CTG GGC ACG-BHQ1
B型	上游引物	TCC TCA ACT CAC TCT TCG AGC G
	下游引物	CGG TGC TCT TGA CCA AAT TGG
	探针	FAM-CCA ATT CGA GCA GCT GAA ACT GCG GTG-BHQ1
A型H1亚型	上游引物	AAC ATG TTA CCC AGG GCA TTT CGC
	下游引物	GTG GTT GGG CCA TGA GCT TTC TTT
	探针	FAM-GAG GAA CTG AGG GAG CAA TTG AGT TCA G-BHQ1
A型H3亚型	上游引物	ACC CTC AGT GTG ATG GCT TCC AAA
	下游引物	TAA GGG AGG CAT AAT CCG GCA CAT
	探针	FAM-ACG CAG CAA AGC CTA CAG CAA CTG T-BHQ1

流感病毒	引物名称	序列（5'→3'）
A型	上游引物	GAC CRA TCC TGT CAC CTC TGA C
	下游引物	GGG CAT TYT GGA CAA AKC GTC TAC G
	探针	FAM- TGC AGT CCT CGC TCA CTG GGC ACG-BHQ1
B型	上游引物	TCC TCA ACT CAC TCT TCG AGC G
	下游引物	CGG TGC TCT TGA CCA AAT TGG
	探针	FAM-CCA ATT CGA GCA GCT GAA ACT GCG GTG-BHQ1
A型H1亚型	上游引物	AAC ATG TTA CCC AGG GCA TTT CGC
	下游引物	GTG GTT GGG CCA TGA GCT TTC TTT
	探针	FAM-GAG GAA CTG AGG GAG CAA TTG AGT TCA G-BHQ1
A型H3亚型	上游引物	ACC CTC AGT GTG ATG GCT TCC AAA
	下游引物	TAA GGG AGG CAT AAT CCG GCA CAT
	探针	FAM-ACG CAG CAA AGC CTA CAG CAA CTG T-BHQ1

临床特征和指标			例（%）
症状			?
?	发热		83（100.0）
?	咳嗽		71（86.5）
?	咯痰		17（20.5）
?	乏力		63（75.9）
?	头痛		52（62.7）
?	关节肌肉疼痛		24（28.9）
?	胸痛		18（21.7）
?	咽喉疼痛		12（14.5）
?	腹泻		6（7.2）
血常规指标			?
?	白细胞计数		?
?	?	升高（>9.5 × 10⁹/L）	6（7.2）
?	?	正常（3.5 × 10⁹/L～9.5 × 10⁹/L）	72（86.7）
?	?	降低（<3.5 × 10⁹/L）	5（6.0）
?	淋巴细胞计数		?
?	?	正常（1.1 × 10⁹/L～3.2 × 10⁹/L）	72（86.7）
?	?	降低（<1.1 × 10⁹/L）	11（13.3）
?	血红蛋白含量（正常值：130 g/L～175 g/L）		83（100.0）
?	红细胞计数（正常值：4.3 × 10¹²/L～5.8 × 10¹²/L）		83（100.0）
?	血小板计数（正常值：125 × 10⁹/L～350 × 10⁹/L）		83（100.0）
生物化学指标			?
?	ALT轻度升高		8（9.6）
?	AST轻度升高		6（7.2）
?	CK轻度升高		3（3.6）
?	LDH轻度升高		3（3.6）
?	肺部CT改变		?
肺部斑片状浸润性阴影			25（30.1）
?	胸膜增厚		21（25.3）

临床特征和指标			例（%）
症状			?
?	发热		83（100.0）
?	咳嗽		71（86.5）
?	咯痰		17（20.5）
?	乏力		63（75.9）
?	头痛		52（62.7）
?	关节肌肉疼痛		24（28.9）
?	胸痛		18（21.7）
?	咽喉疼痛		12（14.5）
?	腹泻		6（7.2）
血常规指标			?
?	白细胞计数		?
?	?	升高（>9.5 × 10⁹/L）	6（7.2）
?	?	正常（3.5 × 10⁹/L～9.5 × 10⁹/L）	72（86.7）
?	?	降低（<3.5 × 10⁹/L）	5（6.0）
?	淋巴细胞计数		?
?	?	正常（1.1 × 10⁹/L～3.2 × 10⁹/L）	72（86.7）
?	?	降低（<1.1 × 10⁹/L）	11（13.3）
?	血红蛋白含量（正常值：130 g/L～175 g/L）		83（100.0）
?	红细胞计数（正常值：4.3 × 10¹²/L～5.8 × 10¹²/L）		83（100.0）
?	血小板计数（正常值：125 × 10⁹/L～350 × 10⁹/L）		83（100.0）
生物化学指标			?
?	ALT轻度升高		8（9.6）
?	AST轻度升高		6（7.2）
?	CK轻度升高		3（3.6）
?	LDH轻度升高		3（3.6）
?	肺部CT改变		?
肺部斑片状浸润性阴影			25（30.1）
?	胸膜增厚		21（25.3）

组别	GP73	IL-6	PCT
流感患者	137.7（100.60，179.40）	12.98（11.28，19.80）	0.032（0.022，0.082）
对照组	32.9（23.00，52.25）	1.52（1.31，1.72）	0.025（0.019，0.035）
F值	12.30	15.61	1.52
P值	<0.001	<0.001	0.253

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