To investigate the distribution of pathogenic bacteria and the risk factors of colonization and infection on refractory wound, and to provide reference for the prevent and treatment of refractory wound infection.

The clinical data of pathogenic bacteria distribution and the risk factors of pathogenic bacteria colonization and infection on refractory wound of burns of surgery inpatients in our hospital from 2012 to 2015 were analyzed, retrospectively.

Total of 265 strains of pathogens were isolated, including 116 strains (43.77%) of Gram-positive and 149 strains (56.23%) of Gram-negatives bacteria. The common pathogenic bacteria were Staphylococcus aureus (104 strains, 39.25%), Pseudomonas aeruginosa (71 strains, 26.79%) and Escherichia coli (28 strains, 10.57%). Totally 177 patients were collected, among whom, 138 patients were with bacteria colonization and infection and 39 patients were without bacteria colonization and infection. Univariate analysis showed that wound area (Z =-3.970, P < 0.001), history of hospitalization (χ² = 5.865, P = 0.015), hospitalization period longer than two weeks (χ² = 5.869, P = 0.0154), lower hemoglobin (χ² = 6.545, P = 0.011), recent implanted catheter (χ² = 5.710, P = 0.017), recent surgery (χ² = 4.320, P = 0.038) and recent infusion (χ² = 10.023, P = 0.002) were all risk factors of positive microorganism culture results. Multivariate Logistic regression analysis indicated that wound area (OR = 1.516, 95%CI: 1.140-2.015, Waldχ² = 8.179, P = 0.004) was an independent risk factor of bacteria colonization and infection.

Gram-negatives bacteria were dominant bacteria on the refractory wound of burns surgery department of our hospital. Wound area was closely related with the pathogenic bacteria colonization and infection.