To investigate the biliary pathogenic distribution and drug susceptibility in patients with biliary tract disease in our district, and to analyze the correlation between the positive rate of germiculture and the influence factors.

Bile specimens collected from 326 patients with biliary tract diseases from January 2010 to December 2015 were cultured and tested for drug sensitivity.

There were 73 and 118 positive samples, respectively, among 140 samples during the first half of this study (2010-2012) and 186 samples during the second half (2013-2015), as well as 85 and 140 strains cultured. Isolated pathogenic bacteria were mainly Gram-negative bacteria, which accounted for 74.1% and 68.6% and Gram-positive accounted for 24.7% and 28.6% during both pariods. Patients who were older, with signs of biliary infection, biliary surgery history, biliary ERCP history, gallstones, benign biliary tract disease and long surgery times were with a higher positive rates. Susceptibility test showed that resistant rates of Gram-negative strains to imipenem and meropenem were the lowest; Gram-negative strains were highly resistant to penicillins, quinolones, some third generation cephalosporins and so on (all > 50.0%). None of Gram-positive strains were resistant to vancomycin, teicoplanin and daptomycin. They were highly resistant to penicillins, quinolones and erythromycin (> 40.0%).

Gram-negative strains were the commonest pathogens in biliary tract disease, which had a high resistant rate to the commonly used antibiotics. The clinical factors of high positive rate of bile bacterial cultur should be paied attention to.