Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolated from a hospital

doi:10.3877/cma.j.issn.1674-1358.2017.02.012

Abstract

Abstract:

Objective

To investigate the molecular epidemiological characteristics of carbapenem-resistant Acinetobacter baumannii (CRAB) isolated from our hospital at a certain time, and to provide the basis for the prevention and control of nosocomial infections.

Methods

Total of 55 stains of CRAB were isolated from the hospital from October 2015 to February 2016. Cloning relationship were conducted by multi-site sequence typing (MLST). The screening of carbapenemase gene and disinfectant gene (qacEΔ1) were detected by PCR.

Results

Six kinds of STs were detected by MLST, which were ST208 (38.2%), ST369 (25.5%), ST195 (20%), ST451 (10.9%), ST195 (3.6%) and ST381 (1.8%). Carbapenemase genes, such as OXA-51 and OXA-23 were both positive, and 96.4% (53/55) of the stains carried qacEΔ1 gene. More than 90% of the specimens were isolated from sputum separated from the patients in Intensive Care Unit.

Conclusions

There was a prevalence caused by CRAB stains of clones during this period. ST208, ST369 and ST195 CRAB carrying OXA-23 gene were the main clones in our hospital.

Key words: Carbapenem-resistant, Acinetobacter baumannii, Multiple-site sequence typing, Carbapenem-resistance gene, Disinfectant-resistant gene

Haiyan Wu, Bin Tan, Zhijun Zhang, Meijie Jiang. Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolated from a hospital[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2017, 11(02): 162-167.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsyhlcgrbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1358.2017.02.012

https://zhsyhlcgrbzz.cma-cmc.com.cn/EN/Y2017/V11/I02/162

Figures/Tables 6

References 24

1	Chuang YC, Sheng WH, Lauderdale TL, et al. Molecular epidemiology, antimicrobial susceptibility and carbapenemase resistance determinants among Acinetobacter baumannii clinical isolates in Taiwan[J]. J Microbiol Immunol Infect,2014,47(4):324-32.
2	汪复，朱德妹，胡付品等. 2012年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志,2013,13(5):321-330.
3	胡付品，朱德妹，汪复, 等. 2013年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志,2014,14(5):369-377.
4	胡付品，朱德妹，汪复, 等. 2014年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志,2015,15(5):401-410.
5	赵书平，牛淑香，宗桂珍. 院内痰培养中常见病原菌的耐药性分析[J/CD]. 中华实验和临床感染病杂志(电子版),2014,8(6):101-102.
6	王伟，毛剑锋，徐伟珍. 大肠埃希菌连续分离株耐药性与季胺类化合物耐药基因研究[J]. 中华医院感染学杂志,2009,19(3):247-249.
7	沈继录，朱德妹，吴卫红, 等. 革兰阴性杆菌碳青霉稀酶产生与细菌耐药性关系的研究[J]. 中华检验医学杂志,2008,31(4):408-414.
8	Brown S, Young HK, Amyes SG. Characterisation of OXA-51, a novel class D carbapenemase found in genetically unrelated clinical strains of Acinetobacter baumannii from Argentina[J]. Clin Microbiol Infect,2005,11(1):15-23.
9	Bartual SG, Seifert H, Hippler C, et al. Development of a multilocus sequence typing scheme for characterization of clinical isolates[J]. J Clin Microbiol,2005,43(9):4382-4390.
10	Dai XT, Sun FJ, Chen ZH, et al. The epidemiology and resistance mechanisms of Acinetobacter baumannii isolates from the respiratory department ICU of a hospital in China[J]. Microb Drug Resist,2014,20(6):618-622.
11	Munoz-Price LS, Arheart K, Nordmann P, et al. Eighteen years of experience with Acinetobacter baumannii in a tertiary care hospital[J]. Crit Care Med,2013,41(12):2733-2742.
12	Gurung M, Rho JS, Lee YC, et al. Emergence and spread of carbapenem-resistant Acinetobacter baumannii sequence type 191 in a Korean hospital[J]. Infect Genet Evol,2013,19(10):219-222.
13	Farshadzadeh Z, Hashemi FB, Rahimi S, et al. Wide distribution of carbapenem resistant Acinetobacter baumannii in burns patients in Iran[J]. Front Microbiol,2015,20(6):1146.
14	Jiang MJ, Liu LJ, Ma YH, et al. Molecular epidemiology of multi-drug Resistant Acinetobacter baumannii isolated in Shandong, China[J]. Front Microbiol,2016,21(7):1687.
15	Ruan Z, Chen Y, Jiang Y, et al. Wide distribution of CC92 carbapenem-resistant and OXA-23-producing Acinetobacter baumannii in multiple provinces of China[J]. Int J Antimicrob Agents,2013,42(4):322-328.
16	Deng M, Zhu MH, Li JJ, et al. Molecular Epidemiology and Mechanisms of Tigecycline Resistance in Clinical Isolates of Acinetobacter baumannii from a Chinese University Hospital[J]. Antimicrob Agents Chemother,2014,58(1):297-303.
17	Liu X, Chen J, Li H, et al. Study of aminoglycoside drug-resistant gene on the plasmid of Acinotobacter baumannii[J]. Chin J Antibiot,2012,37(5),335-337.
18	WangX, Qiao F, Yu R, et al. Clonal diversity of Acinetobacter baumannii clinical isolates revealed by a snapshot study[J]. BMC Microbio,2013,13(1):234.
19	Mosqueda N, Espinal P,Cosgaya C,et al. Nosocomial outbreak of Acinetobacter baumannii producing a plasmid-encoded OXA-23 in Barcelona, Spain[J]. Antimicrob Agents Chemother,2013,57(10):5155-5157.
20	Runnegar N, Sidjabat H, Goh HM, et al. Molecular epidemiology of multidrug-resistant Acinetobacter baumannii in a single institution over a 10-year period[J]. J Clin Microbiol,2010,48(11):4051-4056.
21	Chusri S, Chongsuvivatwong V, Rivera JI, et al. Molecular epidemiology and spatiotemporal analysis of hospital-acquired Acinetobacter baumannii infection in a tertiary care hospital in southern Thailand[J]. J Hosp Infect, 2017,95(1):53-58.
22	Rodriguez CH, Balderrama Yarhui N, Nastro M, et al. Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in South America[J]. J Med Microbiol,2016,65(10):1088-1091.
23	Matsui M, Suzuki S, Suzuki M, et al. Rapid discrimination of Acinetobacter baumannii international clone II lineage by pyrosequencing SNP analyses of bla (OXA-51-like) genes[J]. J Microbiol Methods,2013,94(2):121-124.
24	Zheng YL, Wan YF, Zhou LY, et al. Risk factors and mortality of patients with nosocomial carbapenem-resistant Acinetobacter baumannii pneumonia[J]. Am J Infect Control,2013,41(7):59-63.

抗菌药物	敏感		中介		耐药
抗菌药物	株数	敏感率（%）	株数	中介率（%）	株数	耐药率（%）
阿米卡星	1	1.8	0	0.0	54	98.2
头孢他啶	0	0.0	0	0.0	55	100.0
头孢曲松	0	0.0	0	0.0	55	100.0
环丙沙星	0	0.0	0	0.0	55	100.0
头孢吡肟	0	0.0	0	0.0	55	100.0
庆大霉素	1	1.8	0	0.0	54	98.2
亚胺培南	0	0.0	0	0.0	55	100.0
左氧氟沙星	0	0.0	0	0.0	55	100.0
美罗培南	0	0.0	0	0.0	55	100.0
复方新诺明	2	3.6	0	0.0	53	96.4
哌拉西林/他唑巴坦	0	0.0	0	0.0	55	100.0
妥布霉素	1	1.8	0	0.0	54	98.2
头孢哌酮/舒巴坦	3	5.4	15	27.3	37	67.3
替加环素	41	74.5	14	25.5	0	0.0
多黏菌素B	55	100.0	0	0.0	0	0.0

抗菌药物	敏感		中介		耐药
抗菌药物	株数	敏感率（%）	株数	中介率（%）	株数	耐药率（%）
阿米卡星	1	1.8	0	0.0	54	98.2
头孢他啶	0	0.0	0	0.0	55	100.0
头孢曲松	0	0.0	0	0.0	55	100.0
环丙沙星	0	0.0	0	0.0	55	100.0
头孢吡肟	0	0.0	0	0.0	55	100.0
庆大霉素	1	1.8	0	0.0	54	98.2
亚胺培南	0	0.0	0	0.0	55	100.0
左氧氟沙星	0	0.0	0	0.0	55	100.0
美罗培南	0	0.0	0	0.0	55	100.0
复方新诺明	2	3.6	0	0.0	53	96.4
哌拉西林/他唑巴坦	0	0.0	0	0.0	55	100.0
妥布霉素	1	1.8	0	0.0	54	98.2
头孢哌酮/舒巴坦	3	5.4	15	27.3	37	67.3
替加环素	41	74.5	14	25.5	0	0.0
多黏菌素B	55	100.0	0	0.0	0	0.0

[1]	Jiajin Tu, Wuqiang Liao, Jinjing Liu, Zhipeng Tu, Yuangui Mao. Risk factors and prognosis analysis of Acinetobacter baumannii bloodstream infection in patients with severe burns [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 491-497.
[2]	Wuqiang Liao, Jinjing Liu, Shiqiang Hu, Hongyan Zhang, Yuangui Mao, Jiajin Tu. Research progress on the prevalence of Acinetobacter baumannii in burn patients and its drug resistance mechanism [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(06): 524-530.
[3]	Wenlu Hang, Yongliang Du, Zhanjie Li, Haiquan Li, Jie Zhao, Yu Zhang. Carbapenem-resistant Klebsiella pneumoniae nosocomial infection in a patient with immunodeficiency [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(03): 205-209.
[4]	Fei Lin, Mingyong Yuan, Baodong Ling. Biofilm formation related genes of Acinetobacter baumannii [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(02): 129-132.
[5]	Fenghua Luo, Jianlin Wei. Relationship between the biofilm formation ability of Acinetobacter baumannii and biofilm related genes, drug resistance [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(01): 67-71.
[6]	Xinzhe Liu, Mingxi Hua, Huizhu Wang, Pengcheng Du, Xinmin Xu, Haofeng Xiong, Ang Li, Chen Chen. Whole-genome sequencing analysis on drug resistance and virulence genes of carbapenem-resistant Acinetobacter baumannii [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2020, 14(05): 367-373.
[7]	Haochong Hu, Yiting Liu, Jiayu Guo, Jilin Zou, Zhongbao Chen, Jiangqiao Zhou, Tao Qiu. Treatment and analysis of carbapenem-resistant Klebsiella pneumoniae infection after renal transplantation [J]. Chinese Journal of Transplantation(Electronic Edition), 2023, 17(04): 246-249.
[8]	Wenlei Li, Jushan Wu, Zhe Jia, Na Pan, Wenyue Wu, Binwei Duan, Guangming Li. Risk factors of carbapenem-resistant Klebsiella pneumoniae infection after liver transplantation [J]. Chinese Journal of Transplantation(Electronic Edition), 2021, 15(06): 347-352.
[9]	Dongmei Huang, Jun Liu, Fuxiang Li, Li Fan, Yingjie Jing, Xiao Peng and. Related risk factors of carbapenem-resistant Acinetobacter baumannii in second-level general hospitals of Sichuan province [J]. Chinese Journal of Lung Diseases(Electronic Edition), 2020, 13(03): 355-359.
[10]	Xin Jiang, Chun Pan, Wenhui Huang, Guifen Gan. A study on active CRE screening and CRE infection in anal swabs in Intensive Care Units in Qinghai area [J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2023, 09(02): 178-184.
[11]	Yonghai Liu, Hang Qi, Ting Zhou, Wei Zhang. A case of community-acquired Acinetobacter baumannii pneumonia and a literature review [J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2022, 08(03): 276-280.
[12]	Peiben Liu, Ying Shi, Xiangrong Zuo, Quan Cao. Research progress of Ceftazidime-Avibactam in the treatment of carbapenem-resistant Enterobacteriaceae infection [J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2022, 08(01): 67-73.
[13]	He Sun, Chunfeng He, Xiaodong Wu, Huize Han, Yi Shi, Qiang Li. Efficacy and safety analysis of Ceftazidime-Avibatam in carbapene-resistant Klebsiella pneumoniae infected patients with septic shock [J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2021, 07(01): 76-80.
[14]	Xiaojun Ji, Xiaodong Zhai, Xiaofang Li, Peilin Zhao, Senhui Yue, Yueming Zhang. Therapeutic effect of tigecycline on intracranial infection of extensively drug-resistant Acinetobacter baumannii by dual approach [J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2022, 12(02): 85-89.
[15]	Pingjuan Liu, Kecheng Luo, Jiayin Wu, Kang Liao, Wenwen Hu, Yili Chen. Epidemiological study on intestinal active surveillance of carbapenem-resistant Enterobacteriaceae in neurology intensive care unit [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(04): 235-240.

Please choose a citation manager

Content to export