Genotype analysis of HIV-1 gag genes from cases diagnosed as men who have sex with men in Harbin

doi:10.3877/cma.j.issn.1674-1358.2017.01.006

Abstract

Abstract:

Objective

To investigate the gag genes derived from cases diagnosed as HIV-1-infected men who have sex with men (MSM) in Harbin.

Methods

Genomic DNA were extracted from peripheral blood mononuclear cells (PBMCs) of 49 MSM with HIV-1-infection diagnosed as HIV-1 positive. The gag gene fragments were amplified by nested PCR. Phylogenetic tree was constructed to observe gag clustering and subsequently to analyze the genotyping feature of these cases.

Results

Total of 49 sequences of gag gene were obtained successfully. The phylogenetic analysis showed that these sequences clustered with three subtype lineages as follows: 39 sequences (79.6%) clustered with CRF01_AE lineage, 5 sequences (10.2%) clustered with subtype B, 4 sequences (8.2%) clustered with CRF07_BC. Moreover, one recombinant genotype CRF01B (2.0%) was also identified. And 1 sequence (2.0%) clustered with subtype CRF01B. Among CRF01_AE sequences, 92.3% (36/39) gathered with reference genes from Chinese MSM, and 7.7% (3/39) gathered with reference genes epidemic in Thailand and Southwest regions of China.

Conclusions

The CRF01_AE was the predominant genotype among cases diagnosed as HIV-1-infected MSM in Harbin. The MSM population may become the important source of new genotypes and complicated recombinant forms. Therefore, timely epidemiological surveillance of HIV-1 molecular epidemiology and development of prevention measures for HIV-1 infections among MSM are important.

Key words: Human immunodeficiency virus type 1, Men who have sex with men (MSM), Gag gene, Genotype

Chao Yue, Gang Jin, Haining Li, Bo Song, Yuanlong Lin, Zheng Zhang, Chunying Jin, Yu’ou Li, Yumei Gao, Fuxiang Wang, Shulin Liu, Qinghai Li. Genotype analysis of HIV-1 gag genes from cases diagnosed as men who have sex with men in Harbin[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2017, 11(01): 27-31.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsyhlcgrbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1358.2017.01.006

https://zhsyhlcgrbzz.cma-cmc.com.cn/EN/Y2017/V11/I01/27

Figures/Tables 3

References 23

1	Li X, Xue Y, Cheng H, et al. HIV-1 genetic diversity and its Impact on baseline CD4⁺ T cells and viral loads among recently infected men who have sex with men in Shanghai, China[J]. PLoS One,2015,10(6):e0129559.
2	Shao B, Li Y, Yu L, et al. The HIV/AIDS epidemic characteristics in a northeast province of China--men who have sex with men have made a tremendous contribution to the growth of the HIV epidemic[J]. J Infect,2014,68(3):273-280.
3	Wang K, Yan H, Liu Y, et al. Increasing prevalence of HIV and syphilis but decreasing rate of self-reported unprotected anal intercourse among men who had sex with men in Harbin, China: results of five consecutive surveys from 2006 to 2010[J]. Int J Epidemiol,2012,41(2):423-432.
4	Zhang L, Zhang D, Yu B, et al. Prevalence of HIV infection and associated risk factors among men who have sex with men (MSM) in Harbin, P. R. China[J]. PLoS One,2013,8(3):e58440.
5	Shao B, Li W J, Liu T, et al. Subtype B was the dominant strain among HIV type 1 infections except for the population of men who have sex with men in Harbin City, China[J]. AIDS Res Hum Retroviruses,2013,29(9):1260-1264.
6	孙冬莹，邵冰，张春雷, 等. 哈尔滨市男男同性恋HIV-1感染者gag区基因分析[J/CD]. 中华实验与临床感染病杂志:电子版,2015,9(1):14-17.
7	Feng Y, He X, Hsi JH, et al. The rapidly expanding CRF01_AE epidemic in China is driven by multiple lineages of HIV-1 viruses introduced in the 1990s[J]. AIDS,2013,27(11):1793-1802.
8	Mastro TD, Yip R. The legacy of unhygienic plasma collection in China[J]. AIDS,2006,20(10):1451-1452.
9	Wu Z, Sullivan SG, Wang Y, et al. Evolution of China’s response to HIV/AIDS[J]. Lancet,2007,369(9562):679-690.
10	Xiang H, Hui X, Yuhua R, et al. A comprehensive mapping of HIV-1 genotypes in various risk groups and regions across China based on a nationwide molecular epidemiologic survey[J]. PLoS One,2012,7(10):e47289-e47289.
11	邢辉，潘品良，苏玲, 等. 1996-1998年中国流行的E亚型艾滋病病毒1型毒株的分子流行病学研究[J]. 中国艾滋病性病,2002,8(2):200-203.
12	Li D, Li S, Liu Y, et al. HIV incidence among men who have sex with men in Beijing: a prospective cohort study[J]. BMJ Open,2012,2:e001829.
13	Yuhua R, Jia YJ, Zhang XX, et al. Incidence of HIV-1, syphilis, hepatitis B, and hepatitis C virus infections and predictors associated with retention in a 12-month follow[J]. J AIDS,2009,52(5):604-610.
14	Li D, Yang X, Zhang Z, et al. Nitrite inhalants use and HIV infection among men who have sex with men in China[J]. Biomed Res Int,2014,2014(1):365261.
15	Folch C, Marks G, Esteve A, et al. Factors associated with unprotected sexual intercourse with steady male, casual male, and female partners among men who have sex with men in barcelona, Spain[J]. AIDS Educ Prev,2006,18(3):227-242.
16	Koblin BA, Husnik MJ, Colfax G, et al. Risk factors for HIV infection among men who have sex with men[J]. AIDS,2006,20(5):731-739.
17	Wei H, Su L, Feng Y, et al. Near full-length genomic characterization of a novel HIV type 1 CRF07_ BC/01_AE recombinant in men who have sex with men from Sichuan, China[J]. AIDS Res Hum Retroviruses,2013,29(8):1173-1176.
18	Wang W, Jiang S, Li S, et al. Identification of subtype B, multiple circulating recombinant forms and unique recombinants of HIV type 1 in an MSM cohort in China[J]. AIDS Res Hum Retroviruses,2008,24(10):1245-1254.
19	Feng Y, Li X, Zang X, et al. Identification of a novel HIV-1 second-generation recombinant form (CRF01_AE/CRF07_BC) in Jilin, China[J]. AIDS Res Hum Retroviruses,2014,30(8):819-822.
20	Guo H, Hu H, Zhou Y, et al. A Novel HIV-1 CRF01_AE/B recombinant among men who have sex with men in Jiangsu Province, China[J]. AIDS Res Hum Retroviruses,2014,30(7):706-710.
21	Wu J, Meng Z, Xu J, et al. New emerging recombinant HIV-1 strains and close transmission linkage of HIV-1 strains in the Chinese MSM population indicate a new epidemic risk[J]. PLoS One,2013,8(1):e54322.
22	Wang Y, Liang Y, Yue F, et al. HIV-1 prevalence and subtype/recombinant distribution among travelers entering China from Vietnam at the HeKou port in the Yunnan province, China, between 2003 and 2012[J]. J Med Virol,2015,87(9):1500-1509.
23	Li X, Zang X, Ning C, et al. Molecular epidemiology of HIV-1 in Jilin province, northeastern China: emergence of a new CRF07_BC transmission cluster and intersubtype recombinants[J]. PLoS One,2014,9(10):e110738.

指标		HIV-1基因型				合计（n = 49）
指标		CRF01_AE（n = 39）	Subtype B（n = 5）	CRF07_BC（n = 4）	URF ^a（n = 1）	合计（n = 49）
年龄（岁）
	20～30	11（28.2）	2（40.0）	1（25.0）	0（0.0）	14（28.6）
	31～40	16（41.0）	2（40.0）	1（25.0）	1（100.0）	20（40.8）
	41～50	7（18.0）	1（20.0）	1（25.0）	0（0.0）	9（18.4）
	≥ 51	5（12.8）	0（0.0）	0（0.0）	0（0.0）	5（10.2）
	未知	0（0.0）	0（0.0）	1（25.0）	0（0.0）	1（2.0）
CD4细胞数（个/μl）
	≥ 200	21（53.8）	4（80.0）	2（50.0）	1（100.0）	28（57.1）
	< 200	18（46.2）	1（20.0）	2（50.0）	0（0.0）	21（42.9）

指标		HIV-1基因型				合计（n = 49）
指标		CRF01_AE（n = 39）	Subtype B（n = 5）	CRF07_BC（n = 4）	URF ^a（n = 1）	合计（n = 49）
年龄（岁）
	20～30	11（28.2）	2（40.0）	1（25.0）	0（0.0）	14（28.6）
	31～40	16（41.0）	2（40.0）	1（25.0）	1（100.0）	20（40.8）
	41～50	7（18.0）	1（20.0）	1（25.0）	0（0.0）	9（18.4）
	≥ 51	5（12.8）	0（0.0）	0（0.0）	0（0.0）	5（10.2）
	未知	0（0.0）	0（0.0）	1（25.0）	0（0.0）	1（2.0）
CD4细胞数（个/μl）
	≥ 200	21（53.8）	4（80.0）	2（50.0）	1（100.0）	28（57.1）
	< 200	18（46.2）	1（20.0）	2（50.0）	0（0.0）	21（42.9）

[1]	Pei Qian, Ying Bao, Huimei Huang, Yan Han, Lei Suo, Nan Yang, Xiaomin An, Jiawen Dang. Clinical phenotypes and genotypes analysis of PKHD1 gene variants in children with autosomal recessive polycystic kidney disease [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2022, 18(05): 540-547.
[2]	Lifang Wang, Pingshan Pan, Dahua Meng, Li Lin, Yangjin Zuo, Xiaoxia Qiu. Prenatal diagnosis of 440 fetuses with hemoglobin H disease in Guangxi area [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2021, 17(01): 75-80.
[3]	Juan Du, bo Song, Xiaoming Yan, Jiaming Niu, Yuanlong Lin, Xiaohong Chen, Jin Li, Han Liang, Zheng Zhang, Yuou Li, Fuxiang Wang, Bing Shao. Quantitative results of human immunodeficiency virus total deoxyribonucleic acid and ribonucleic acid in peripheral blood mononuclear cells to distinguish the activity of viral transcription [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(03): 165-171.
[4]	Xuan Zheng, Bao Zhang, Shilong Li, Xiaoru Zhang. Characteristics of drug resistance mutation sites in reverse transcription polymerase region and influencing factors of drug resistance in 150 patients with different genotypes of chronic hepatitis B [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(02): 82-89.
[5]	Linong Yao, Qunying Lu, Wei Ruan, Zhe Zhan, Shuirong Zhu, Liping Jiang. Gene genotypes and variation of 5 cases with Orientia tsutsugamushi infection [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2020, 14(02): 110-116.
[6]	Chuangjie Mao, Rong Hu, Yilan Zeng, Qiong Zhang, Xintong Kang. Efficacy of tenofovir disoproxil on blocking mother-to-child transmission of hepatitis B virus with high viral load of women in the middle of pregnancy in Sichuan region [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(06): 485-490.
[7]	Juanping Yu, Qi Wei, Qianqian Wang, Zhongbao Chang, Hong Ye, Qinhua Xu, Xiaohua Li. Genotypes distribution of human papillomavirus in infected cervical tissue from 17 160 women for physical examination in Anhui area [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(05): 389-395.
[8]	Xiaoman Zhang, Tao Xu, Zhengju Xu. Characteristics of genotypes and resistant mutations within hepatitis B virus (HBV) reverse transcriptase sequences of nucleos(t)ide analogues experienced patients with HBV infection in southern Minnan region [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(04): 293-299.
[9]	Dongxiao Liu, Yongbin Ding, Liuqun Shan, Gongming Cheng. Association between rs2230724 gene polymorphism of tyrosine kinase 2 and risk of colorectal cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2022, 16(06): 667-671.
[10]	Siyuan Chen, Xiarong Hu, Chuping Xie. Clinical and prognostic significance of mutant-allele tumor heterogeneity in thyroid papillary carcinoma [J]. Chinese Journal of Clinicians(Electronic Edition), 2019, 13(11): 832-836.
[11]	Tingting Yu, Dong Pu, Dongling Li, Hongying Wang, Runwu Zhang, Caimei Ding, Lihua Li, jing Bai, Xiaofei Li. Analysis of genotype and PIVKA-II level of Patients with HBV-related hepatocellular carcinoma in Kunming [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2020, 08(03): 166-169.
[12]	Qian Kong, Guannan Bai, Yu Zhou, Bo Yan. Correlation between CTSB gene promoter region polymorphism and clinical features in patients with acute myocardial infarction [J]. Chinese Journal of Diagnostics(Electronic Edition), 2022, 10(03): 171-176.
[13]	Aiping Ju, Yanxia Liu, Keng Lin, Xiangrong Meng, Xichong Li, Guoxiang Wei, Shuxian Liu. Genotypes and hematological characteristics of population with αβ-thalassemia in the northern of Guangzhou [J]. Chinese Journal of Diagnostics(Electronic Edition), 2020, 08(02): 121-125.
[14]	Liying Zhuang, Qilun Lai, Lu Liu, Yue Lou, Shanhu Xu, Yu Jin, Xiaoli Liu. The predictive value of Framingham risk score for mild cognitive impairment progress to dementia in middle-aged and elderly patients [J]. Chinese Journal of Geriatrics Research(Electronic Edition), 2021, 08(03): 20-23.
[15]	Huimin Chen, Yilong Wang. Update on association among cerebral small vessel disease, APOE genotype and related protein [J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2020, 14(01): 40-46.

Please choose a citation manager

Content to export