PFGE analysis and antimicrobial resistance of E. coli isolated from intra-abdominal infection

doi:10.3877/cma.j.issn.1674-1358.2016.05.010

Abstract

Abstract:

Objective

To study the relationship of E.coli strains isolated from intra-abdominal infection, and the relationship of homology and the antimicrobial susceptibility results of different strains.

Methods

DNA of strains were digested with XbaⅠ restriction enzyme, then were typed by pulsed field gel electrophoresis (PFGE). PFGE patterns were analyzed with Bionumerrics software. The antimicrobial susceptibility results of E. coli strains and the clinical information of patients were collected.

Results

The homology of strains were from 60.4% to 97.4%, 6 among 47 strains were above 85%. One episode of SBP could be a result of reinfection caused by one PFGE type strain, or more than one PFGE type strains, also could be a recurrent infection of the last episode. Antimicrobial susceptibility results of more than 15 antibiotics were indentical among strains with genetic similarity ≥ 85%.

Conclusions

Most strains isolated from intra-abdominal infection were different. Strains with genetic similarity ≥ 85% have identical or similar antimicrobial susceptibility .

Key words: Pulsed field gel electrophoresis (PFGE), E. coli, Ascites, Resistance

Yuzhi Li, Xiaoli Du, Zhihai Chen. PFGE analysis and antimicrobial resistance of E. coli isolated from intra-abdominal infection[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(05): 559-564.

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References 21

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