Association of chemokine (C-C motif) receptor 6 gene polymorphisms with susceptibility to rheumatoid arthritis in Hanzhong Area of Shanxi Province

doi:10.3877/cma.j.issn.1674-1358.2016.02.013

Abstract

Abstract:

Objectives

To assess the association of chemokine (C-C motif ) receptor 6 (CCR6) gene polymorphisms with rheumatoid arthritis (RA) among ethnic Han Chinese in Hanzhong Area of Shanxi Province.

Methods

The data of 768 patients with RA and 960 healthy controls were analyzed by case-control association method. All individuals were collected in Hanzhong Area of Shanxi Province of Han Chinese descent. Five (rs1331301, rs1556413, rs3093024, rs1854853 and rs3093023) single nucleotide polymorphisms (SNP) in CCR6 gene region were genotyped by the Snapshot method.

Results

There were a significant association of the rs3093024 [A] Allele (OR = 1.22, P = 0.003) and dominant model (OR = 1.33, P = 0.004) with RA susceptibility with Hanzhong of ShanXi Han Chinese population. The rs1854853 [A] Allele (OR = 1.20, P = 0.007) and Dominant model (OR = 1.35, P = 0.006) frequency distribution were significantly different between RA group and normal control group. Equally important, rs3093023 [T] Allele (OR = 1.26, P = 0.009), dominant model (OR = 1.38, P = 0.002) and recessive model (OR = 1.33, P = 0.025) frequency distribution were significantly different between RA group and normal control group. The rs1331301 and rs1556413 allele and genotyped polymorphisms were not significantly associated with RA susceptibility.

Conclusions

CCR6 gene of rs3093024, rs1854853 and rs3093023 are significantly associated with rheumatoid arthritis of Han Chinese population in Hanzhong Area of Shanxi Province.

Key words: Chemokine (C-C motif) receptor 6 (CCR6) gene, Single nucleotide polymorphism, Rheumatoid arthritis

Mingfeng Yan, Xiaobo Li, Yang Su, Ting Li, Miao Chen, Yang Chai. Association of chemokine (C-C motif) receptor 6 gene polymorphisms with susceptibility to rheumatoid arthritis in Hanzhong Area of Shanxi Province[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2016, 10(02): 193-199.

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References 21

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分组	例数	性别（男/女）	年龄（±s，岁）	患病时间（±s，年）	RF+ [例（%）]	抗-CCP阳性[例（%）]
RA组	768	247/521	49.5 ± 12.3	3.81 ± 2.9	643（83.7）	526（68.5）
对照组	960	375/585	48.9 ± 12.8	—	—	—
统计量		χ² = 6.612^b	t = 0.551^a	—	—	—
P值		0.011	0.547	—	—	—

分组	例数	性别（男/女）	年龄（±s，岁）	患病时间（±s，年）	RF+ [例（%）]	抗-CCP阳性[例（%）]
RA组	768	247/521	49.5 ± 12.3	3.81 ± 2.9	643（83.7）	526（68.5）
对照组	960	375/585	48.9 ± 12.8	—	—	—
统计量		χ² = 6.612^b	t = 0.551^a	—	—	—
P值		0.011	0.547	—	—	—

分组	例数	性别（男/女）	年龄（±s，岁）	患病时间（±s，年）
ACCP⁺ RA组	526	155/371	47.7 ± 10.2	3.81 ± 2.9
对照组	960	375/585	48.9 ± 12.8	—
统计量		χ² = 13.634^b	t=3.824^a	—
P值		0.000	0.044	—

分组	例数	性别（男/女）	年龄（±s，岁）	患病时间（±s，年）
ACCP⁺ RA组	526	155/371	47.7 ± 10.2	3.81 ± 2.9
对照组	960	375/585	48.9 ± 12.8	—
统计量		χ² = 13.634^b	t=3.824^a	—
P值		0.000	0.044	—

SNP		引物序列（5'→3'）	退火温度（℃）	产物大小（bp）
rs1331301	正向	GCAGATGGCTTGGGTTTAG	58	800
	反向	GAGGTCGTCATCCTGGTAG
	SnapShot	ACTAAGGAATGATAATATTCTGAAATTGTC
rs1556413	正向	AGCCGTTGGCGTTTCCTAT	58	690
	反向	ACTGCTGCCTCCTTCACCC
	SnapShot	CCTAAGTGGAGAAGGTAACCTAGATTTCCCCTTAAATTAC
rs3093024	正向	CCCTGTGACCTGCTCTTCT	58	479
	反向	CCATTTACTTCCCACCTTTT
	SnapShot	GGAGCTCCTCTGTGCTCCCTCCTGTGAACTGGACGCTCGC
rs1854853	正向	AAGGTGGGAAGTAAATGGC	58	503
	反向	TGGAGGGACAGGAAGAAGA
	SnapShot	CCAGCTACTT TATGGGGTAG
rs3093023	正向	TCTGCCTGGAAACTTATTG	58	369
	反向	GGATGGAGATTGAGACCCT
	SnapShot	TATTGAAACTTCCTCAAATTTAAAATCACA

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