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中华实验和临床感染病杂志(电子版) ›› 2017, Vol. 11 ›› Issue (02) : 185 -189. doi: 10.3877/cma.j.issn.1674-1358.2017.02.017

临床论著

胃癌患者幽门螺杆菌感染与蛋白磷酸酶2A的癌性抑制因子表达增强的相关性
陈飞1, 李俊,1   
  1. 1. 432000 孝感市,武汉科技大学附属孝感医院肿瘤科,孝感市中心医院肿瘤科
  • 收稿日期:2016-02-20 出版日期:2017-04-15
  • 通信作者: 李俊

Correlation of Helicobacter pylori infection and cancer ousinhibitor protein phosphatase 2A enhanced expression in patients with gastric cancer

Fei Chen1, Jun Li,1   

  1. 1. Department of Oncology, The Affiliated Xiaogan Hospital of Wuhan University of Science and Technolog, Xiaogan 432000, China; Department of Oncology, Xiaogan Central Hospital, Xiaogan 432000, China
  • Received:2016-02-20 Published:2017-04-15
  • Corresponding author: Jun Li
引用本文:

陈飞, 李俊. 胃癌患者幽门螺杆菌感染与蛋白磷酸酶2A的癌性抑制因子表达增强的相关性[J/OL]. 中华实验和临床感染病杂志(电子版), 2017, 11(02): 185-189.

Fei Chen, Jun Li. Correlation of Helicobacter pylori infection and cancer ousinhibitor protein phosphatase 2A enhanced expression in patients with gastric cancer[J/OL]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2017, 11(02): 185-189.

目的

分析胃癌患者幽门螺杆菌增强蛋白磷酸酶2A的癌性抑制因子(CIP2A)的表达,以期为临床治疗提供理论依据。

方法

随机选取2012年10月至2015年9月本院确诊的胃癌患者共80例,通过对幽门螺杆菌及胃癌细胞培养,采用实时荧光定量法及Western blot法分别检测CIP2A蛋白表达水平,分析胃癌组织中CIP2A表达与幽门螺杆菌感染及临床病理特征相关性,应用Cox比例风险回归模型进行患者预后因素分析。

结果

CIP2A蛋白在胃癌组织、癌旁组织、正常胃黏膜组织中阳性表达率依次为66.3%、25.0%和10.0%,组间差异具有统计学意义(χ2= 37.29、P = 0.025)。CIP2A蛋白在肿瘤浸润程度加深、淋巴结转移的胃癌组织中表达更高;胃癌患者组幽门螺杆菌感染率(67.5%)显著高于健康对照组(40.0%),差异具有统计学意义(χ2 = 5.136、P = 0.023);CIP2A蛋白在无幽门螺杆菌感染组和幽门螺杆菌感染组胃癌患者中阳性表达率分别为11.5%和88.9%,两组CIP2A蛋白表达率差异具有统计学意义(χ2 = 8.428、P = 0.005)。胃癌组织的淋巴结转移状况、肿瘤浸润深度及CIP2A蛋白表达量高低等临床病理特征和患者预后显著相关。

结论

胃癌患者幽门螺杆菌感染率高于健康人群;CIP2A蛋白阳性表达是影响胃癌患者预后的独立因素。

Objective

To investigate the mechanism of Helicobacter pylori (HP) enhancing the expression of cancer ousinhibitor protein phosphatase 2A (CIP2A) in patients with gastric cancer, and to provide theoretical basis for clinical treatment.

Methods

Total of 80 patients with gastric cancer in our hospital from October 2012 to September 2015 were collected. HP and the gastric cancer cell from tissue samples were cultured, and the changes of CIP2A protein expression were detected by real-time fluorescence quantitative method and Western blot. The correlation of CIP2A expression in gastric cancer tissue and clinicopathological features of Hp infection were analyzed. The prognostic factors of patients with gastric cancer were analyzed by Cox proportional hazards regression model.

Results

The positive expression rates of CIP2A protein in gastric cancer tissues, paracancerous tissues and normal gastric mucosal tissues were 66.3%, 25% and 10%, respectively, with significant difference (χ2 = 37.29, P = 0.025). The expression levels of CIP2A protein in gastric cancer tissue of tumor infiltrating extent and lymph node metastasis were significantly high. The infection rate of HP in patient of gastric cancer group (67.5%) was significantly higher than that of the control group (40.0%), with significant difference (χ2= 5.136, P = 0.023). The positive expression rate of CIP2A protein were 11.5% and 88.9% in gastric cancer group with non-HP infection and with HP infection, with significant difference (χ2 = 8.428, P = 0.005). The prognosis of patients were significantly correlated with the clinical pathological features which include lymph node metastasis, tumor infiltration depth and expression of CIP2A protein in gastric cancer tissue.

Conclusions

HP infection rate of patients with gastric cancer was higher than that of the normal population; CIP2A protein expression was an independent prognostic factor in patients with gastric cancer.

表1 CIP2A蛋白表达与胃癌患者临床病理特征的相关性[例(%)]
表2 Cox比例风险回归单因素预后分析
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