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中华实验和临床感染病杂志(电子版)

中华实验和临床感染病杂志(电子版) ›› 2017, Vol. 11 ›› Issue (01) : 27 -31. doi: 10.3877/cma.j.issn.1674-1358.2017.01.006

临床论著

哈尔滨市男男同性恋HIV-1感染者gag基因分型特征分析
岳超1, 金刚1, 李海宁1, 宋波2, 林元龙2, 张铮3, 金春英3, 李宇欧3, 高玉梅3, 王福祥2, 刘树林1,†(), 李庆海1   
  1. 1. 150081　哈尔滨市，哈尔滨医科大学基因组中心
    2. 150001　哈尔滨市，哈尔滨医科大学附属第四医院感染科
    3. 150030　哈尔滨市，哈尔滨市传染病医院重症医学科
  • 收稿日期:2016-02-06 出版日期:2017-02-15
  • 通信作者: 刘树林
  • 基金资助:
    黑龙江省自然科学基金(No. H201383); 黑龙江省大学生创业创新基金(No. 201510226017、No. 201410226040)

Genotype analysis of HIV-1 gag genes from cases diagnosed as men who have sex with men in Harbin

Chao Yue1, Gang Jin1, Haining Li1, Bo Song2, Yuanlong Lin2, Zheng Zhang3, Chunying Jin3, Yu’ou Li3, Yumei Gao3, Fuxiang Wang2, Shulin Liu1,†(), Qinghai Li1   

  1. 1. Genomics Research Center (One of The State-Province Key Laboratory of Biopharmaceutical Engineering), Harbin Medical University, Harbin 150081, China
    2. Department of Infectious Diseases, Fourth Af?liated Hospital of Harbin Medical University, Harbin 150001, China
    3. Intensive Care Unit, Infectious Diseases Hospital of Harbin, Harbin 150030, China
  • Received:2016-02-06 Published:2017-02-15
  • Corresponding author: Shulin Liu
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引用本文：

岳超, 金刚, 李海宁, 宋波, 林元龙, 张铮, 金春英, 李宇欧, 高玉梅, 王福祥, 刘树林, 李庆海. 哈尔滨市男男同性恋HIV-1感染者gag基因分型特征分析[J]. 中华实验和临床感染病杂志(电子版), 2017, 11(01): 27-31.

Chao Yue, Gang Jin, Haining Li, Bo Song, Yuanlong Lin, Zheng Zhang, Chunying Jin, Yu’ou Li, Yumei Gao, Fuxiang Wang, Shulin Liu, Qinghai Li. Genotype analysis of HIV-1 gag genes from cases diagnosed as men who have sex with men in Harbin[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2017, 11(01): 27-31.

目的

分析哈尔滨市确诊的男男同性恋（MSM）HIV-1感染病例gag基因序列，确定新发感染毒株基因型。

方法

收集2014年7月至12月确诊且未经过抗病毒治疗的感染者抗凝血，分离外周血单个核细胞，从中提取基因组DNA，采用巢式PCR法扩增gag基因。采用MEGA 5.05软件构建gag基因系统发育树，分析基因型特点。

结果

共收集49例MSM感染者样本，且均获得gag基因序列。系统发育树显示，49例gag基因主要分布于3个亚型内，其中CRF01_AE亚型39例，占79.6%；B亚型5例，占10.2%；CRF07_BC亚型4例，占8.2%。另外，发现CRF01B重组型1例，占2.0%。在CRF01_AE毒株中，92.3%（36/39）与我国MSM人群参考株成簇，7.7%（3/39）与泰国及我国西南地区异性传播参考株成簇。

结论

本研究时间段哈尔滨市确诊MSM感染病例以CRF01_AE基因型为主。MSM人群可能成为本地区复杂基因型的重要来源，实时监测该人群新发感染病例的分子流行病学特征，对制定有效地预防和干预措施非常重要。

关键词: Ⅰ型人类免疫缺陷病毒, 男男同性恋, gag基因, 基因型
Objective

To investigate the gag genes derived from cases diagnosed as HIV-1-infected men who have sex with men (MSM) in Harbin.

Methods

Genomic DNA were extracted from peripheral blood mononuclear cells (PBMCs) of 49 MSM with HIV-1-infection diagnosed as HIV-1 positive. The gag gene fragments were amplified by nested PCR. Phylogenetic tree was constructed to observe gag clustering and subsequently to analyze the genotyping feature of these cases.

Results

Total of 49 sequences of gag gene were obtained successfully. The phylogenetic analysis showed that these sequences clustered with three subtype lineages as follows: 39 sequences (79.6%) clustered with CRF01_AE lineage, 5 sequences (10.2%) clustered with subtype B, 4 sequences (8.2%) clustered with CRF07_BC. Moreover, one recombinant genotype CRF01B (2.0%) was also identified. And 1 sequence (2.0%) clustered with subtype CRF01B. Among CRF01_AE sequences, 92.3% (36/39) gathered with reference genes from Chinese MSM, and 7.7% (3/39) gathered with reference genes epidemic in Thailand and Southwest regions of China.

Conclusions

The CRF01_AE was the predominant genotype among cases diagnosed as HIV-1-infected MSM in Harbin. The MSM population may become the important source of new genotypes and complicated recombinant forms. Therefore, timely epidemiological surveillance of HIV-1 molecular epidemiology and development of prevention measures for HIV-1 infections among MSM are important.

Key words: Human immunodeficiency virus type 1, Men who have sex with men (MSM), Gag gene, Genotype
表1 哈尔滨市确诊的19例感染HIV-1 MSM人口学和流行性信息[例（%）]
指标 HIV-1基因型 合计（n = 49）
CRF01_AE（n = 39） Subtype B（n = 5） CRF07_BC（n = 4） URF a（n = 1）
年龄（岁）          
  20～30 11（28.2） 2（40.0） 1（25.0） 0（0.0） 14（28.6）
  31～40 16（41.0） 2（40.0） 1（25.0） 1（100.0） 20（40.8）
  41～50 7（18.0） 1（20.0） 1（25.0） 0（0.0） 9（18.4）
  ≥ 51 5（12.8） 0（0.0） 0（0.0） 0（0.0） 5（10.2）
  未知 0（0.0） 0（0.0） 1（25.0） 0（0.0） 1（2.0）
CD4细胞数（个/μl）          
  ≥ 200 21（53.8） 4（80.0） 2（50.0） 1（100.0） 28（57.1）
  < 200 18（46.2） 1（20.0） 2（50.0） 0（0.0） 21（42.9）
图1 哈尔滨市HIV-1感染MSM的gag基因系统进化分析
图2 HRB14046 gag基因重组分析
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