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中华实验和临床感染病杂志(电子版)

中华实验和临床感染病杂志(电子版) ›› 2026, Vol. 20 ›› Issue (01) : 10 -14. doi: 10.3877/cma.j.issn.1674-1358.2026.01.003

综述

幽门螺杆菌耐药与毒力基因分子检测方法研究进展
兰庆站1,2, 李波清2, 牟彤3, 卢志明1, 金炎1, 邵春红1,†()   
  1. 1　250014　济南市，山东第一医科大学附属省立医院临床医学检验部
    2　264003　烟台市，滨州医学院基础医学院病原生物学教研室
    3　250014　济南市，山东第一医科大学附属省立医院消化内科
  • 收稿日期:2025-07-17 出版日期:2026-02-15
  • 通信作者: 邵春红
  • 基金资助:
    山东省自然科学基金项目(ZR2021MH214，ZR2025MS1331)

Research progress on molecular detection methods for resistance and virulence genes of Helicobacter pylori

Qingzhan Lan1,2, Boqing Li2, Tong Mu3, Zhiming Lu1, Yan Jin1, Chunhong Shao1,†()   

  1. 1 Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250014, China
    2 Department of Pathogenic Biology, School of Basic Medical Sciences, Binzhou Medical University, Yantai 264003, China
    3 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250014, China
  • Received:2025-07-17 Published:2026-02-15
  • Corresponding author: Chunhong Shao
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兰庆站, 李波清, 牟彤, 卢志明, 金炎, 邵春红. 幽门螺杆菌耐药与毒力基因分子检测方法研究进展[J/OL]. 中华实验和临床感染病杂志(电子版), 2026, 20(01): 10-14.

Qingzhan Lan, Boqing Li, Tong Mu, Zhiming Lu, Yan Jin, Chunhong Shao. Research progress on molecular detection methods for resistance and virulence genes of Helicobacter pylori[J/OL]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2026, 20(01): 10-14.

幽门螺杆菌是引起人体消化道疾病最重要的病原菌之一，已被世界卫生组织列为Ⅰ类致癌物，其精准检测对临床诊疗具有重要意义。目前，临床常规诊断方法众多，以血清学抗体检测法、13C/14C尿素呼气试验法、微需氧培养法以及组织学染色法等常用，然而随着抗菌药物的过度使用，其耐药现象日益严重，传统检测方法已不能完全满足个体化诊疗需求，分子检测技术通过靶向检测耐药与毒力基因已成为临床诊疗与科研攻关迫切需求的重要工具，为临床提供了新的解决方案。本文对幽门螺杆菌分子检测方法研究进展进行综述，探讨其临床应用价值及技术局限性，为幽门螺杆菌感染个体化精准诊疗方案的选择提供理论依据。

关键词: 幽门螺杆菌, 耐药基因, 分子检测, 实时荧光定量聚合酶链式反应, 全基因组测序

Helicobacter pylori is one of the most important pathogens causing human gastrointestinal diseases and has been classified as a Group 1 carcinogen by the World Health Organization (WHO). Accurate detection of Helicobacter pylori is of great significance for clinical diagnosis and treatment. Currently, a variety of routine clinical diagnostic methods are available, with serological antibody detection, 13C/14C urea breath test (UBT), microaerophilic culture and histological staining being the most commonly used. However, due to the overuse of antibacterial agents, Helicobacter pylori drug resistance has become increasingly prevalent, rendering traditional detection methods inadequate to fully meet the demands of personalized medicine. Molecular detection technologies, which target drug resistance and virulence genes, have emerged as crucial tools urgently required for clinical practice and scientific research, providing novel solutions for clinical management. This review summarizes the research progress of molecular detection methods for Helicobacter pylori, discusses their clinical application value and technical limitations, and aims to provide a theoretical basis for the selection of individualized and precise diagnosis and treatment strategies for Helicobacter pylori infection.

Key words: Helicobacter pylori, Drug resistance genes, Molecular detection, Real-time fluorescent quantitative polymerase chain reaction, Genome sequencing
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