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中华实验和临床感染病杂志(电子版) ›› 2018, Vol. 12 ›› Issue (01) : 94 -97. doi: 10.3877/cma.j.issn.1674-1358.2018.01.019

所属专题： 文献；

基础论著

人脐带间充质干细胞对耐亚胺培南铜绿假单胞菌生长的抑制作用
刘晓虹1, 杨浩鸣2, 郑璇儿1, 杨淑梅1, 杨杰1,†()   
  1. 1. 510000　广州市，广东省妇幼保健院新生儿科；519000　珠海市，珠海市人民医院新生儿科
    2. 510000　广州市，广东省妇幼保健院新生儿科
  • 收稿日期:2017-02-27 出版日期:2018-02-15
  • 通信作者: 杨杰
  • 基金资助:
    广州市科学研究专项项目(No. 2060404)

Inhibitory effect of human umbilical cord mesenchymal stem cells (hUCMSCs) against Pseudomonas aeruginosa

Xiaohong Liu1, Haoming Yang2, Xuaner Zheng1, Shumei Yang1, Jie Yang1,†()   

  1. 1. Department of Neonatology, Guangdong Women and Children’s Hospital, Guangzhou 510000, China
  • Received:2017-02-27 Published:2018-02-15
  • Corresponding author: Jie Yang
  • About author:
    Corresponding author: Yang Jie, Email:
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引用本文：

刘晓虹, 杨浩鸣, 郑璇儿, 杨淑梅, 杨杰. 人脐带间充质干细胞对耐亚胺培南铜绿假单胞菌生长的抑制作用[J]. 中华实验和临床感染病杂志(电子版), 2018, 12(01): 94-97.

Xiaohong Liu, Haoming Yang, Xuaner Zheng, Shumei Yang, Jie Yang. Inhibitory effect of human umbilical cord mesenchymal stem cells (hUCMSCs) against Pseudomonas aeruginosa[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2018, 12(01): 94-97.

目的

探讨人脐带间充质干细胞（hUCMSCs）是否抑制耐亚胺培南铜绿假单胞菌（IRPA）的生长。

方法

将IRPA菌液加入hUCMSCs细胞悬液中设为实验组，将IRPA菌液加入成纤维细胞细胞悬液中为对照组，共培育6 h后采用平板计数法对两组IRPA进行计数并比较；采用蛋白免疫印记技术和酶联免疫法检测两组共培育上清液中抗菌肽LL-37（Cathelicidin/LL-37）及人β防御素2（HBD-2）。

结果

实验组中的细菌集落数（CFU）低于对照组，差异具有统计学意义 [（2.63 ± 0.58）× 106 CFU/ml vs.（3.69 ± 0.91）× 106 CFU/ml，t = 19.93、P = 0.031]。实验组共培育液中抗菌肽LL-37和HBD-2浓度高于对照组，差异具有统计学意义[抗菌肽LL-37：（7.99 ± 0.45）ng/ml vs. （0.18 ± 0.04）ng/ml，t = 78.30、P = 0.007；HBD-2：（249.38 ± 14.19）pg/ml vs.（1.00 ± 0.58）pg/ml，t = 78.23、P = 0.009]。

结论

人脐带间充质干细胞对耐亚胺培南铜绿假单胞菌生长起抑制作用，其可能通过分泌抗菌肽LL-37和HBD-2来实现。

关键词: 人脐带间充质干细胞, 耐亚胺培南铜绿假单胞菌, 抗菌肽LL-37, 人β防御素2
Objective

To investigate inhibitory effect of the human umbilical cord mesenchymal stem cells (hUCMSCs) on the growth of imipenem-resistant Pseudomonas aeruginosa (IRPA).

Methods

IRPA added into hUCMSCs were collected as the experimental group; while the IRPA co-cultured with fibroblast were taken as the control group. After 6 hours of co-cultivation, colony forming units (CFUs) of IRPA were counted in each group using plate counting method. Then the levels of Cathelicidin/LL-37 and human β-defensin 2 (HBD-2) in cultured medium were detected by Western-blot and ELISA.

Results

The CFUs of IRPA in experimental group were less than those in control groups, with significant differences (2.63 ± 0.58) × 106 CFU/ml vs. (3.69 ± 0.91) × 106 CFU/ml; t = 19.93, P < 0.05). Cathelicidin/LL-37 and hBD2 in experimental group were higher than those of experimental group [Cathelicidin/LL-37: (7.99 ± 0.45) ng/ml vs. (0.18 ± 0.04) ng/ml; t = 78.30, P = 0.007; HBD-2: (249.38 ± 14.19) pg/ml vs. (1.00 ± 0.58) pg/ml; t = 78.23, P = 0.009].

Conclusions

hUCMSCs plays an inhibitory role on the growth of IRPA. The possible mechanism is that hUCMSCs could produce antimicrobial peptide: Cathelicidin/LL-37 and hBD2.

Key words: Human umbilical cord mesenchymal stem cells, Imipenem-resistant Pseudomonas aeruginosa, Cathelicidin/LL-37, Human β-defensin 2
图1 Western-blot检测两组的LL-37的蛋白表达
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