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中华实验和临床感染病杂志(电子版) ›› 2021, Vol. 15 ›› Issue (02) : 129 -132. doi: 10.3877/cma.j.issn.1674-1358.2021.02.010

所属专题: 文献

短篇论著

鲍曼不动杆菌生物膜相关基因研究
蔺飞1, 袁明勇1, 凌保东2,()   
  1. 1. 610500 成都市,成都医学院第一附属医院药学部
    2. 610500 成都市,成都医学院结构特异性小分子药物研究四川省高校重点实验室
  • 收稿日期:2020-04-29 出版日期:2021-04-15
  • 通信作者: 凌保东
  • 基金资助:
    国家自然科学基金委项目(No. 81373454)

Biofilm formation related genes of Acinetobacter baumannii

Fei Lin1, Mingyong Yuan1, Baodong Ling2,()   

  1. 1. Department of Pharmacy, The First Affiliated Hospital of Chengdu Medical College, Chengdu, China
    2. Sichuan Province College Key Laboratory of Structure-Speci?c Small Molecule Drugs, School of Pharmacy, Chengdu Medical College, Chengdu, China
  • Received:2020-04-29 Published:2021-04-15
  • Corresponding author: Baodong Ling
引用本文:

蔺飞, 袁明勇, 凌保东. 鲍曼不动杆菌生物膜相关基因研究[J/OL]. 中华实验和临床感染病杂志(电子版), 2021, 15(02): 129-132.

Fei Lin, Mingyong Yuan, Baodong Ling. Biofilm formation related genes of Acinetobacter baumannii[J/OL]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(02): 129-132.

目的

探讨临床分离鲍曼不动杆菌生物膜相关基因的不同生物膜形成能力。

方法

收集成都医学院第一附属医院2014年1月至2015年1月住院患者临床分离的鲍曼不动杆菌47株,采用结晶紫染色法测定鲍曼不动杆菌的生物膜形成能力。PCR检测abaI、bap、pgaA、pgaB、pgaC、pgaD、csuA/B、csuA、csuB、csuC、csuD、csuE、bfmR、bfmS、pglC、pglL、gacS、H-NS和ompA等19个生物膜相关基因。采用Pearson卡方检验或连续校正卡方检验分析生物被膜形成能力相关基因检出率。

结果

47株菌株中生物膜形成菌株13株;共检测到17个生物膜相关基因。基因ompA、pgaC、pgaD和bfmR检出率均为100%(47/47),pglL和H-NS检出率均为0(0/47),bap检出率为17.02%(8/47),csuD检出率为53.19%(25/47),其余基因检出率> 85%(40/47)。基因bap、pgaB、csuA/B、csuA、csuB和csuD在生物膜阳性菌株中的检出率分别为53.85%、92.31%、100%、100%、92.31%和53.85%,于阴性菌株中检出率分别为2.94%、85.29%、91.18%、97.06%、88.24%和52.94%;csuA/B和csuA在生物膜阳性菌株检出率均为100%,在阴性菌株中检出率分别为91.18%和97.06%。基因bap在生物膜阳性菌株(53.85%、7/13)和阴性菌株的检出率(2.94%、1/34)差异有统计学意义(χ2 = 13.838、P < 0.001),而其他基因在阳性和阴性菌株中检出率差异无统计学意义(P均> 0.05)。

结论

鲍曼不动杆菌生物被膜相关基因bap可能促进生物膜的形成。

Objective

To investigate the different biofilm formation ability of clinical isolation of Acinetobacter baumannii (A. baumannii) biofilm related genes.

Methods

A total of 47 A. baumannii clinical isolates from January 2014 to January 2015 in the First Affiliated Hospital of Chengdu Medical College were collected to analyze the biofilm formation by crystal violet staining assay. The biofilm genes abaI, bap, pgaA, pgaB, pgaC, pgaD, csuA/B, csuA, csuB, csuC, csuD, csuE, bfmR, bfmS, pglC, pglL, gacS, H-NS and ompA were detected by polymerase chain reaction. The clinical isolates biofilm formation ability elated genes were analyzed by Pearson Chi-square to analysis or Chi-square test with continuous correction.

Results

Thirteen isolates exhibited biofilm formation ability among 47 clinical isolates, and seventeen genes were detected. The detection rates of gene ompA、pgaC、pgaD and bfmR were all 100% (47/47), respectively. The detection rates of pglL and H-NS were both 0 (0/47), the ratios of bap and csuD were 17.02% (8/47) and 53.19% (25/47), respectively. The detection rate of other gene was > 85% (40/47). The detection rates of gene bap, pgaB, csuA/B, csuA, csuB and csuD were 53.85%, 92.31%, 100%, 100%, 92.31% and 53.85% in positive strains, respectively, which were 2.94%, 85.29%, 91.18%, 97.06%, 88.24% and 52.94%, respectively in negative strains. The detection rate of csuA/B and csuB were both 100% in positive strains and were 91.18% and 97.06% in negative isolates, respectively. The detection rates of bap gene were significantly different between positive (53.85%, 7/13) and negative (2.94%, 1/34) strains, with significant difference (χ2 = 13.838, P < 0.001), but there was no significant difference in the detection rates of other genes between positive and negative strains (all P > 0.05).

Conclusion

A. baumannii biofilm related gene bap may promote the ability of biofilm formation.

表1 基因引物序列及目的产物长度
表2 鲍曼不动杆菌生物膜相关基因检出率[株(%)]
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