探讨流式细胞技术在高危16型、18型人乳头状瘤病毒（HPV）感染诊断中的临床价值。

选取2013年1月至2017年1月于深圳市人民医院筛查的200例女性作为研究对象，征得所有受试者知情同意后对其行流式细胞技术诊断和荧光定量聚合酶链式反应（FQ-PCR）诊断，比较两种方法的诊断价值。

两种诊断方法下正常者、宫颈上皮内瘤变（CIN）Ⅰ期者16型和18型HPV E6蛋白阳性率差异无统计学意义（P均> 0.05）；流式细胞技术诊断CIN Ⅱ期、Ⅲ期、宫颈癌者阳性率低于荧光PCR诊断，差异有统计学意义（P均< 0.001）。流式细胞技术诊断≥ CIN Ⅱ宫颈病变的敏感度、特异度和准确率分别为87.5%（35/40）、98.2%（157/160）和96.0%（192/200）；荧光定量PCR诊断≥ CIN Ⅱ宫颈病变的敏感度、特异度和准确率分别为85.0%（34/40）、96.9%（155/160）和94.5%（189/200），差异均无统计学意义（P均> 0.05）。

流式细胞技术操作简便，可有效避免样品交叉感染所致的假阳性，提高HPV感染诊断的准确性。

To investigate the diagnosis value of flow cytometry (FCM) in type 16 and type 18 of human papillomavirus (HPV) infection.

Total of 200 women screened in People’s Hospital of Shenzhen from January 2013 to January 2017 were selected as the research objects, all the subjects were informed with the consent, and were diagnosed by FCM and fluorescent quantitative polymerase chain reaction (FQ-PCR). The diagnosis results of the above two methods were compared.

There was no significant difference in the positive rates of E6 protein between type 16 and type 18 HPV among normal patients and patients in stage Ⅰ with cervical intraepithelial neoplasia (CIN) (all P > 0.05 ). The positive rates of HPV detected by FCM in patients with CIN Ⅱ, Ⅲ and cervical cancer were lower than that of the diagnosis of FCM, with significant differences (all P < 0.001). The sensitivity, specific and accurate rates in the diagnosis of patients ≥ CIN Ⅱ cervical lesion degree detected by FCM were 87.5% (35/40), 98.2% (157/160) and 96.0% (192/200), respectively. The sensitivity, specific and accurate rates in the diagnosis of patients ≥ CIN Ⅱ cervical lesion degree detected by FQ-PCR were 85.0% (34/40), 96.9% (155/160) and 94.5% (189/200), respectively. There was no significant differences between the two diagnostic methods (all P > 0.05).

FCM is easy to operate, which could effectively avoid the false positive caused by cross-infection of samples and improve the diagnosis accuracy of HPV infection.